D1 receptors regulate dendritic morphology in normal and stressed prelimbic cortex.
D1 receptors regulate dendritic morphology in normal and stressed prelimbic cortex.
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DOI：
10.1016/j.psyneuen.2014.09.020
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发表时间：
2015-01
影响因子：
3.7
通讯作者：
Wellman, Cara L.
中科院分区：
文献类型：
--
作者：
Una, Grant L.;Borders, Candace B.;Lundewall, Leslie J.;Wellman, Cara L.
压力和前额叶皮质功能障碍都与心理障碍有关，而内侧前额叶皮质（mPFC）的结构和功能会因压力而改变。慢性约束应激引起大鼠mPFC边缘前区树突状回缩。应激时，mPFC中的多巴胺释放增加，长期服用多巴胺能激动剂导致树突重塑。因此，应激诱导的多巴胺能传递的改变可能有助于树突重塑。我们检测了多巴胺D1受体（D1R）在日常约束应激下对PL树突形态的影响。大鼠接受每日约束应激（3小时/天，10天）或保持无应激。在每一组中，大鼠在受限前每天向PL中输注D1R拮抗剂SCH23390或载药；没有接受过手术的大鼠和有压力的大鼠也接受了检查。在约束的最后一天，对大鼠实施安乐死，并对大脑进行高尔基体组织学处理。重建PL锥体神经元，量化树突形态。与非应激大鼠相比，注入车辆的应激大鼠表现出树突缩回，而PL中的D1R阻断阻止了这种作用。此外，在非应激大鼠中，D1R阻断产生树突缩回。这些影响不是由于HPA轴对急性应激反应的衰减：在另一组接受急性约束应激的大鼠中，有或没有D1R阻断的血浆皮质酮水平没有显著差异。这些发现表明，应激时mPFC中的多巴胺能传递直接导致应激诱导的顶端树突缩回，而无应激时多巴胺传递对维持正常树突形态很重要。
Both stress and dysfunction of prefrontal cortex are linked to psychological disorders, and structure and function of medial prefrontal cortex (mPFC) are altered by stress. Chronic restraint stress causes dendritic retraction in the prelimbic region (PL) of mPFC in rats. Dopamine release in mPFC increases during stress, and chronic administration of dopaminergic agonists results in dendritic remodeling. Thus, stress-induced alterations in dopaminergic transmission in PL may contribute to dendritic remodeling. We examined the effects of dopamine D1 receptor (D1R) blockade in PL during daily restraint stress on dendritic morphology in PL. Rats either underwent daily restraint stress (3 h/day, 10 days) or remained unstressed. In each group, rats received daily infusions of either the D1R antagonist SCH23390 or vehicle into PL prior to restraint; unstressed and stressed rats that had not undergone surgery were also examined. On the final day of restraint, rats were euthanized and brains were processed for Golgi histology. Pyramidal neurons in PL were reconstructed and dendritic morphology was quantified. Vehicle-infused stressed rats demonstrated dendritic retraction compared to unstressed rats, and D1R blockade in PL prevented this effect. Moreover, in unstressed rats, D1R blockade produced dendritic retraction. These effects were not due to attenuation of the HPA axis response to acute stress: plasma corticosterone levels in a separate group of rats that underwent acute restraint stress with or without D1R blockade were not significantly different. These findings indicate that dopaminergic transmission in mPFC during stress contributes directly to the stress-induced retraction of apical dendrites, while dopamine transmission in the absence of stress is important in maintaining normal dendritic morphology.
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影响因子：
10.6
作者：
Cools, Roshan;D'Esposito, Mark
通讯作者：
D'Esposito, Mark
影响因子：
34.7
作者：
Arnsten, Amy F. T.
通讯作者：
Arnsten, Amy F. T.
影响因子：
3.3
作者：
Ball, K. T.;Wellman, C. L.;Miller, B. R.;Rebec, G. V.
通讯作者：
Rebec, G. V.
DOI：
10.1073/pnas.0908563106
发表时间：
2009-10-20
影响因子：
11.1
作者：
Hains, Avis Brennan;Vu, Mai Anh T.;Arnsten, Amy F. T.
通讯作者：
Arnsten, Amy F. T.
影响因子：
3
作者：
GLASER, EM;VANDERLOOS, H
通讯作者：
VANDERLOOS, H