Diversity and characteristics of colonization of root-associated fungi of Vaccinium uliginosum.
Diversity and characteristics of colonization of root-associated fungi of Vaccinium uliginosum.
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越橘根部伴生真菌的多样性及定植特征
DOI：
10.1038/s41598-018-33634-1
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发表时间：
2018-10-16
影响因子：
4.6
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Yang H;Zhao X;Liu C;Bai L;Zhao M;Li L
本研究调查了越橘在中国主要野生分布范围内的丛状菌根真菌 (EMF) 多样性。使用 Illumina MiSeq 测序分析 V. uliginosum 毛根中的真菌群落。只有 22 个 OTU 被分配到假定的 EMF 属。根霉属（Rhizoscyphus）和蜜霉属（Meliniomyces）在 EMF 群落中占主导地位，其次是棒孢菌属（Clavaria）、奇木霉属（Oidiodendron）、毛毛霉属（Lachnum）、无头霉属（Acephala）和花头霉属（Phialocephala）。大兴安岭地区的深色有隔内生菌 (DSE) 读数多于其他研究区，与放大交叉法测定 DSE 定植百分比的结果相似。总体而言，高通量测序数据提供了 V. uliginosum 根相关真菌的粗略群落规模草图。从 V. uliginosum 根块中分离出 280 个生长缓慢的菌株，根据形态学和内转录间隔序列比较，这些菌株与 16 个真菌属相匹配。隐孢子虫 ericae、Oidiodendron maius、Lachnum sp.、Sordariomycetes sp. 和 Pleosporales sp. 的分离株通过再合成轨迹形成了环状菌丝卷。通过台盼蓝染色观察发根中 EMF 和 DSE 的共存。提出了 V. uliginosum 毛根中 EMF 和 DSE 共存的假定模型。我们认为，在某些环境胁迫下，例如低温和可利用养分匮乏，杜鹃花植物可能有利于 DSE 和 EMF 的共同定植。
This study investigated ericoid mycorrhizal fungi (EMF) diversity in Vaccinium uliginosum across its main wild distribution range in China. Fungal communities in hair roots of V. uliginosum were analyzed using Illumina MiSeq sequencing. Only 22 OTUs were assigned to putative EMF genera. Rhizoscyphus and Meliniomyces dominated EMF communities, followed by Clavaria, Oidiodendron, Lachnum, Acephala, and Phialocephala. There were more dark septate endophytes (DSE) reads from the Greater Khingan Mountains than from other study areas, similar to the results of the percent colonization of DSE by the magnified intersections method. Overall, high-throughput sequencing data provided a rough community-scale sketch of root-associated fungi of V. uliginosum. Two hundred and eighty slow-growing isolates were isolated from root pieces of V. uliginosum, and the isolates matched 16 fungal genera on the basis of morphological and internal transcribed spacer sequence comparison. The isolates of Cryptosporiopsis ericae, Oidiodendron maius, Lachnum sp., Sordariomycetes sp., and Pleosporales sp., formed ericoid hyphal coils via resynthesis trails. The co-existence between EMF and DSE in hair roots was observed via trypan blue staining. A putative model for the co-existence between EMF and DSE in the hair roots of V. uliginosum was proposed. We suggest that under certain environmental stresses, such as low temperature and poor available nutrients, ericoid plants may favor co-colonization by both DSE and EMF.
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影响因子：
9.4
作者：
Grelet, Gwen-Aelle;Johnson, David;Anderson, Ian C.
通讯作者：
Anderson, Ian C.
影响因子：
3.9
作者：
Barrow, JR;Aaltonen, RE
通讯作者：
Aaltonen, RE
影响因子：
8.3
作者：
AJMAL, M;KHAN, MA
通讯作者：
KHAN, MA
影响因子：
9.4
作者：
DALPE, Y
通讯作者：
DALPE, Y
DOI：
10.1139/cjb-76-7-1205
发表时间：
1998-07-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
Jumpponen, A;Trappe, JM
通讯作者：
Trappe, JM