Aspergillus section Nidulantes (formerly Emericella): Polyphasic taxonomy, chemistry and biology.

Aspergillus section Nidulantes (formerly Emericella): Polyphasic taxonomy, chemistry and biology.
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DOI:
10.1016/j.simyco.2016.10.001
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发表时间:
2016-06
影响因子:
16.5
通讯作者:
Samson, R. A.
Samson, R. A.
中科院分区:
生物学1区
文献类型:
--
作者:
Chen, A. J.;Frisvad, J. C.;Sun, B. D.;Varga, J.;Kocsube, S.;Dijksterhuis, J.;Kim, D. H.;Hong, S. -B.;Houbraken, J.;Samson, R. A.

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长柄曲霉包括具有显著形态特征的种类,如双孢分生孢子,柄呈棕色,如果存在,子囊孢子的产生嵌入大量的h<s:1> lle细胞,通常带有红棕色的子囊孢子。在双名命名系统中,本节大多数种具有有性状态,被命名为Emericella。本研究利用内转录间隔区(ITS)、部分β-微管蛋白(BenA)、钙调蛋白(CaM)和RNA聚合酶II第二大亚基(RPB2)序列对该菌株进行了多位点分子系统发育分析。在绵绵亚属中有9个分支,包括新分支洞穴属。采用形态特征、外向性、生理特征和系统发育的多相方法研究了小棱属植物的分类。在此基础上,将Nidulantes组划分为7个分支和65种,其中10种为新种。子囊孢子的颜色、形状、大小和纹饰、分生孢子和囊泡的形状和大小、生长温度等形态特征是鉴别物种的重要依据。菊科植物的许多种类产生致癌真菌毒素sterigmatocystin。最重要的真菌毒素是黄曲霉毒素、甾体半胱氨酸、回曲素、复曲霉毒素、星形毒素和帕罗西林,而其他外源性毒素是有用的药物或候选药物,如棘白菌素、mulundocandins、calbistrins、变三醇、变多酚和terrain。黄曲霉毒素B1由四种黄曲霉产生:A. astellatus、A. miraensis、A. olivicola和A. venezuelan。
Aspergillus section Nidulantes includes species with striking morphological characters, such as biseriate conidiophores with brown-pigmented stipes, and if present, the production of ascomata embedded in masses of Hülle cells with often reddish brown ascospores. The majority of species in this section have a sexual state, which were named Emericella in the dual name nomenclature system. In the present study, strains belonging to subgenus Nidulantes were subjected to multilocus molecular phylogenetic analyses using internal transcribed spacer region (ITS), partial β-tubulin (BenA), calmodulin (CaM) and RNA polymerase II second largest subunit (RPB2) sequences. Nine sections are accepted in subgenus Nidulantes including the new section Cavernicolus. A polyphasic approach using morphological characters, extrolites, physiological characters and phylogeny was applied to investigate the taxonomy of section Nidulantes. Based on this approach, section Nidulantes is subdivided in seven clades and 65 species, and 10 species are described here as new. Morphological characters including colour, shape, size, and ornamentation of ascospores, shape and size of conidia and vesicles, growth temperatures are important for identifying species. Many species of section Nidulantes produce the carcinogenic mycotoxin sterigmatocystin. The most important mycotoxins in Aspergillus section Nidulantes are aflatoxins, sterigmatocystin, emestrin, fumitremorgins, asteltoxins, and paxillin while other extrolites are useful drugs or drug lead candidates such as echinocandins, mulundocandins, calbistrins, varitriols, variecolins and terrain. Aflatoxin B1 is produced by four species: A. astellatus, A. miraensis, A. olivicola, and A. venezuelensis.
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