Proteomic profiling for the identification of serum diagnostic biomarkers for abdominal and thoracic aortic aneurysms.
Proteomic profiling for the identification of serum diagnostic biomarkers for abdominal and thoracic aortic aneurysms.
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DOI：
10.1186/1477-5956-11-27
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发表时间：
2013-06-27
期刊：
影响因子：
2
通讯作者：
Matsumoto K
中科院分区：
文献类型：
--
作者：
Satoh K;Maniwa T;Oda T;Matsumoto K
主动脉瘤是一种越来越常见的血管疾病，具有致命的影响。然而，除了根据囊肿大小进行诊断外，还没有确定的诊断。为此，主动脉瘤的血清蛋白生物标志物是有价值的。虽然大多数关于血清生物标志物发现的研究是基于患者组与健康组的血清蛋白的比较，但我们认为，比较一名患者的一系列蛋白质谱，如术前和术后血清中的蛋白质谱，将有助于生物标志物的鉴定，因为一名患者内的一系列蛋白质谱的变异性小于组间的变异性。在这项研究中，我们检查了腹主动脉瘤（AAA）和胸主动脉瘤（TAA）患者手术后与手术前相比具有差异水平的血清蛋白，以确定AAA和TAA的潜在血清生物标志物。使用了具有用于相对和绝对定量（iTRAQ）标记的同量异位素标签的蛋白质组学方法，随后是纳米液相色谱（nanoLC）-基质辅助激光解吸电离（MALDI）-飞行时间（TOF/TOF）-串联质谱（MS/MS）。在AAAs和TAAs患者的血清中，共有63和71个具有差异水平的蛋白进一步缩小到6和8个增加的蛋白（术后与术前的比值为1.3倍）（p < 0.05，患者与对照），12和17种蛋白质减少（< 0.77倍，术后对比术前）（p < 0.05，患者对比对照）。AAA和TAA患者术后血清中所有增加的蛋白质包括几种已知的急性期蛋白质。另一方面，在减少的蛋白质中，我们发现了有趣的分子，如α-2-巨球蛋白，凝溶胶蛋白，kallistatin等，其中，我们证实了kallistatin在AAA和TAA患者和TAA患者的α-2-巨球蛋白在AAA和TAA患者术后血清中的水平与对照血清中的水平相似。综上所述，我们的研究结果表明，Kallistatin和α-2-巨球蛋白分别是AAA和TAA和TAA的潜在血清生物标志物。
Aortic aneurysm is an increasingly common vascular disorder with fatal implication. However, there is no established diagnosis other than that based on aneurysmal size. For this purpose, serum protein biomarkers for aortic aneurysms are valuable. Although most of the studies on serum biomarker discovery have been based on comparison of serum proteins from the patient group with those from the healthy group, we considered that comparison of serial protein profiles such as those in presurgical and postsurgical sera within one patient would facilitate identification of biomarkers since the variability of serial protein profiles within one patient is smaller than that between groups. In this study, we examined serum proteins with differential levels in postsurgery compared with those in presurgery after the removal of aneurysmal tissues in abdominal aortic aneurysm (AAA) and thoracic aortic aneurysm (TAA) patients in order to identify potential serum biomarkers for AAAs and TAAs. A proteomic approach with an isobaric tag for relative and absolute quantitation (iTRAQ) labeling followed by nano liquid chromatography (nanoLC)-matrix-assisted laser desorption ionization (MALDI)-time of flight (TOF/TOF)-tandem mass spectrometry (MS/MS) was used. In the sera of patients with AAAs and TAAs, a total of 63 and 71 proteins with differential levels were further narrowed down to 6 and 8 increased proteins (≧1.3 fold, postsurgical vs. presurgical) (p < 0.05, patient vs. control) and 12 and 17 decreased proteins (< 0.77 fold, postsurgical vs. presurgical) (p < 0.05, patient vs. control) in postsurgical sera compared with those in presurgical sera, respectively. All of the increased proteins in postsurgical sera of both AAA and TAA patients included several known acute-phase proteins. On the other hand, in the decreased proteins, we found intriguing molecules such as α-2-macroglobulin, gelsolin, kallistatin, and so on. Among them, we confirmed that kallistatin in both AAA and TAA patients and α-2-macroglobulin in TAA patients showed decrease levels in postsurgical sera similar to those in control sera by Western blot analysis with other sera from AAA and TAA patients. Taken together, our findings suggest that Kallistatin and α-2-macroglobulin are potential serum biomarkers for both AAA and TAA and TAA, respectively.
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影响因子：
3.4
作者：
Qian, WJ;Jacobs, JM;Smith, RD
通讯作者：
Smith, RD
影响因子：
4.1
作者：
DAVEAU, M;ROUET, P;SALIER, JP
通讯作者：
SALIER, JP
DOI：
10.2174/187152508785909537
发表时间：
2008-10-01
影响因子：
--
作者：
Correale, Michele;Brunetti, Natale Daniele;Di Biase, Matteo
通讯作者：
Di Biase, Matteo
影响因子：
5
作者：
Gao, Lin;Yin, Hang;Chao, Julie
通讯作者：
Chao, Julie
影响因子：
3.5
作者：
CASTELL, JV;GOMEZLECHON, MJ;HEINRICH, PC
通讯作者：
HEINRICH, PC