A guide to super-resolution fluorescence microscopy.
A guide to super-resolution fluorescence microscopy.
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DOI:
10.1083/jcb.201002018
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发表时间:
2010-07-26
期刊:
影响因子:
--
通讯作者:
Leonhardt H
中科院分区:
文献类型:
--
作者:
Schermelleh L;Heintzmann R;Leonhardt H
For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new super-resolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new approaches have created unprecedented new possibilities to investigate the structure and function of cells.
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