Insight into differential responses of upland and paddy rice to drought stress by comparative expression profiling analysis.
Insight into differential responses of upland and paddy rice to drought stress by comparative expression profiling analysis.
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通过比较表达谱分析深入了解旱稻和水稻对干旱胁迫的差异反应。
DOI：
10.3390/ijms14035214
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发表时间：
2013-03-04
影响因子：
5.6
通讯作者：
Xiong L
中科院分区：
文献类型：
--
作者：
Ding X;Li X;Xiong L
以旱稻IRAT109和珍汕97 （ZS97）为研究对象，在形态、生理和转录水平上对旱稻和水稻的干旱响应进行了系统比较。IRAT109在叶片卷曲、根系体积、叶片失水比和相对电导率等抗旱相关性状上表现较好。在转录水平上，IRAT109在干旱早期受干旱诱导的基因较多，而ZS97在不同干旱程度下具有动态表达模式的基因较多。干旱条件下，IRAT109中与生殖发育和定位建立相关的基因被抑制较多，而ZS97中与细胞组分降解相关的基因被诱导较多。通过对位于14个QTL区间的36个干旱响应基因在ZS97、IRAT109和QTL区间近等基因系（near isogenic lines, NILs）中的表达模式和表达水平进行分析，发现与ZS97和IRAT109相比，半数以上的干旱响应基因在近等基因系中的表达模式或表达水平发生了变化。本研究结果可为剖析抗旱性性状的遗传基础，以及缩小抗旱性性状的候选基因范围提供有价值的信息。
In this study, the drought responses of two genotypes, IRAT109 and Zhenshan 97 (ZS97), representing upland and paddy rice, respectively, were systematically compared at the morphological, physiological and transcriptional levels. IRAT109 has better performance in traits related to drought avoidance, such as leaf rolling, root volumes, the ratio of leaf water loss and relative conductivity. At the transcriptional level, more genes were induced by drought in IRAT109 at the early drought stage, but more genes had dynamic expression patterns in ZS97 at different drought degrees. Under drought conditions, more genes related to reproductive development and establishment of localization were repressed in IRAT109, but more genes involved in degradation of cellular components were induced in ZS97. By checking the expression patterns of 36 drought-responsive genes (located in 14 quantitative trail loci [QTL] intervals) in ZS97, IRAT109 and near isogenic lines (NILs) of the QTL intervals, we found that more than half of these genes had their expression patterns or expression levels changed in the NILs when compared to that in ZS97 or IRAT109. Our results may provide valuable information for dissecting the genetic bases of traits related to drought resistance, as well as for narrowing the candidate genes for the traits.
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