Ultrasound Improved the Non-Covalent Interaction of β-Lactoglobulin with Luteolin: Regulating Human Intestinal Microbiota and Conformational Epitopes Reduced Allergy Risks.
Ultrasound Improved the Non-Covalent Interaction of β-Lactoglobulin with Luteolin: Regulating Human Intestinal Microbiota and Conformational Epitopes Reduced Allergy Risks.
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超声波改善β-乳球蛋白与木犀草素的非共价相互作用：调节人体肠道微生物群和构象表位降低过敏风险。
DOI：
10.3390/foods11070988
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发表时间：
2022-03-29
期刊：
影响因子：
--
通讯作者：
Tu Z
中科院分区：
文献类型：
--
作者：
Wang T;Chen W;Shao Y;Liu J;Tu Z
本研究旨在探讨超声波对β-乳球蛋白（β-LG）和木犀草素（LUT）非共价相互作用的影响，以及过敏原性与人体肠道菌群的关系。处理后，β-LG的构象发生了变化，表现为α-螺旋含量、内源荧光强度和表面疏水性降低，而β-折叠含量增加。分子对接研究表明，β-LG与LUT之间存在氢键、货车der Walls键和疏水键等非共价相互作用。超声处理后的β-LG-LUT复合物通过改变β-LG-LUT的构象表位，降低IgG/IgE结合能力，抑制KU 812细胞的过敏反应;同时，β-LG-LUT复合物影响人体肠道菌群的组成，如双歧杆菌和普氏菌的相对丰度。因此，超声改善了β-LG与LUT的非共价相互作用，β-LG致敏性的降低依赖于构象表位和人体肠道菌群的变化。
The present study aims to investigate the effects of ultrasound on the non-covalent interaction of β-lactoglobulin (β-LG) and luteolin (LUT) and to investigate the relationship between allergenicity and human intestinal microbiota. After treatment, the conformational structures of β-LG were changed, which reflected by the decrease in α-helix content, intrinsic fluorescence intensity and surface hydrophobicity, whereas the β-sheet content increased. Molecular docking studies revealed the non-covalent interaction of β-LG and LUT by hydrogen bond, van der Walls bond and hydrophobic bond. β-LG-LUT complex treated by ultrasound has a lower IgG/IgE binding ability and inhibits the allergic reaction of KU812 cells, depending on the changes in the conformational epitopes of β-LG. Meanwhile, the β-LG-LUT complex affected the composition of human intestinal microbiota, such as the relative abundance of Bifidobacterium and Prevotella. Therefore, ultrasound improved the non-covalent interaction of β-LG with LUT, and the reduction in allergenicity of β-LG depends on conformational epitopes and human intestinal microbiota changes.
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DOI：
10.3390/foods11010122
发表时间：
2022-01-04
期刊：
Foods (Basel, Switzerland)
影响因子：
--
作者：
Chavan P;Sharma P;Sharma SR;Mittal TC;Jaiswal AK
通讯作者：
Jaiswal AK
影响因子：
12.4
作者：
Savage JH;Lee-Sarwar KA;Sordillo J;Bunyavanich S;Zhou Y;O'Connor G;Sandel M;Bacharier LB;Zeiger R;Sodergren E;Weinstock GM;Gold DR;Weiss ST;Litonjua AA
通讯作者：
Litonjua AA
表征 β 乳球蛋白和选定酚酸之间的非共价相互作用
影响因子：
10.7
作者：
Li, Xin;Dai, Taotao;Li, Ti
通讯作者：
Li, Ti
影响因子：
3
作者：
He, Shengfa;Li, Xin;Chen, Hongbing
通讯作者：
Chen, Hongbing
影响因子：
10.7
作者：
Cao, Yanyun;Xiong, Youling L.;True, Alma D.
通讯作者：
True, Alma D.