A Consensus Model of Homology-Directed Repair Initiated by CRISPR/Cas Activity.
A Consensus Model of Homology-Directed Repair Initiated by CRISPR/Cas Activity.
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DOI：
10.3390/ijms22083834
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发表时间：
2021-04-07
影响因子：
5.6
通讯作者：
Rivera-Torres N
中科院分区：
文献类型：
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作者：
Bloh K;Rivera-Torres N
自ssODN定向基因编辑的作用机制自诞生以来一直是CRISPR基因编辑领域讨论的话题。已经发现了多种可比较但不同的 DNA 修复途径，无论有或没有修复模板寡核苷酸。我们之前描述了寡核苷酸驱动的 DNA 修复的 ExACT 途径，该途径由两步 DNA 合成驱动的修复组成，由双链断裂上游和下游的修复寡核苷酸 (ssODN) 同时结合催化。为了更好地阐明基于 ExACT 的修复机制，我们用其他类似的非基于 ssODN 的 DNA 修复机制中的概述挑战了该途径的假设。 ExACT 途径的这一更全面的迭代更好地描述了 CRISPR 切割后在修复寡核苷酸存在的情况下 DNA 修复发生的多种不同方式，以及这些先前不同的途径如何重叠并导致更独特的修复结果。
The mechanism of action of ssODN-directed gene editing has been a topic of discussion within the field of CRISPR gene editing since its inception. Multiple comparable, but distinct, pathways have been discovered for DNA repair both with and without a repair template oligonucleotide. We have previously described the ExACT pathway for oligo-driven DNA repair, which consisted of a two-step DNA synthesis-driven repair catalyzed by the simultaneous binding of the repair oligonucleotide (ssODN) upstream and downstream of the double-strand break. In order to better elucidate the mechanism of ExACT-based repair, we have challenged the assumptions of the pathway with those outlines in other similar non-ssODN-based DNA repair mechanisms. This more comprehensive iteration of the ExACT pathway better described the many different ways where DNA repair can occur in the presence of a repair oligonucleotide after CRISPR cleavage, as well as how these previously distinct pathways can overlap and lead to even more unique repair outcomes.
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作者：
Brandsma I;Gent DC
通讯作者：
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影响因子：
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作者：
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