Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster.
Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster.
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DOI：
10.3390/jdb11010002
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发表时间：
2022-12-23
影响因子：
2.7
通讯作者：
Olesnicky, Eugenia C.
中科院分区：
文献类型：
--
作者：
Tixtha, Erika J.;Super, Meg K.;Titus, M. Brandon;Bono, Jeremy M.;Olesnicky, Eugenia C.
RNA 结合蛋白 (RBP) 在种系和神经系统内基因表达的转录后调控中发挥着重要作用。与不孕症和神经系统疾病有关的 RBP 编码基因突变的普遍性强调了这一点。我们之前描述了高度保守的 RBP Caper 在果蝇幼虫外周系统神经突形态发生和运动行为中的作用。然而，虽然雀跃功能在胚胎发生过程中在许多不同的组织类型中广泛表达，但尚未在神经系统之外进行研究。在这里，我们描述了刺山柑在生育和交配行为中的新作用。我们发现 Caper 在整个卵子发生过程中在卵泡中表达，但对于卵室的正确形成是可有可无的。此外，通过遗传损伤或 RNA 干扰介导的雌性种系中刺山柑的敲低，刺山柑功能降低，导致雌性产卵量明显少于对照同类。此外，这种表型随着年龄的增长而加剧。刺山柑功能障碍还会导致部分胚胎和幼虫死亡。鉴于刺山柑在后生动物中高度保守，这些发现也可能与脊椎动物相关。
RNA binding proteins (RBPs) play a fundamental role in the post-transcriptional regulation of gene expression within the germline and nervous system. This is underscored by the prevalence of mutations within RBP-encoding genes being implicated in infertility and neurological disease. We previously described roles for the highly conserved RBP Caper in neurite morphogenesis in the Drosophila larval peripheral system and in locomotor behavior. However, caper function has not been investigated outside the nervous system, although it is widely expressed in many different tissue types during embryogenesis. Here, we describe novel roles for Caper in fertility and mating behavior. We find that Caper is expressed in ovarian follicles throughout oogenesis but is dispensable for proper patterning of the egg chamber. Additionally, reduced caper function, through either a genetic lesion or RNA interference-mediated knockdown of caper in the female germline, results in females laying significantly fewer eggs than their control counterparts. Moreover, this phenotype is exacerbated with age. caper dysfunction also results in partial embryonic and larval lethality. Given that caper is highly conserved across metazoa, these findings may also be relevant to vertebrates.
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DOI：
10.1073/pnas.92.22.10114
发表时间：
1995-10-24
影响因子：
11.1
作者：
HERNDON, LA;WOLFNER, MF
通讯作者：
WOLFNER, MF
影响因子：
4.5
作者：
Ameku T;Niwa R
通讯作者：
Niwa R
影响因子：
4.3
作者：
Lin WH;Giachello CN;Baines RA
通讯作者：
Baines RA
DOI：
10.1261/rna.048298.114
发表时间：
2015-06
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Kanke M;Jambor H;Reich J;Marches B;Gstir R;Ryu YH;Ephrussi A;Macdonald PM
通讯作者：
Macdonald PM
影响因子：
--
作者：
Ceron, Julian;Rual, Jean-Francois;Chandra, Abha;Dupuy, Denis;Vidal, Marc;van den Heuvel, Sander
通讯作者：
van den Heuvel, Sander