oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction.
oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction.
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DOI：
10.1261/rna.048298.114
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Macdonald PM
中科院分区：
文献类型：
--
作者：
Kanke M;Jambor H;Reich J;Marches B;Gstir R;Ryu YH;Ephrussi A;Macdonald PM
果蝇奥斯卡（osk） mRNA是不寻常的，因为它同时具有编码和非编码功能。作为信使rna， osk编码胚胎模式和生殖细胞形成所需的蛋白质。与此功能无关，osk mRNA的缺失破坏了核体的形成，并阻碍了卵子发生的进展。在这里，我们发现osk mRNA的缺失也会影响调节蛋白的分布，使它们与种系内大RNPs的关联减弱，并允许它们在体细胞卵泡细胞中积累。osk mRNA的这一和其他非编码功能是由多个具有不同作用的序列元件介导的。osk 3 ' UTR两个不同区域的众多结合位点提供的一个作用是隔离翻译调节因子布鲁诺（Bru），它本身控制着osk mRNA的翻译。这定义了一个新的监管回路，Bru限制osk的活动，而osk反过来限制Bru的活动。其他不与Bru结合的功能元件位于RNA的3 '端附近，在卵母细胞中起作用，是必不可少的。尽管不同类型的元素对RNA的功能有不同的作用，但任何突变都会导致卵泡细胞中生殖系调节因子的积累。
The Drosophila oskar (osk) mRNA is unusual in that it has both coding and noncoding functions. As an mRNA, osk encodes a protein required for embryonic patterning and germ cell formation. Independent of that function, the absence of osk mRNA disrupts formation of the karyosome and blocks progression through oogenesis. Here we show that loss of osk mRNA also affects the distribution of regulatory proteins, relaxing their association with large RNPs within the germline, and allowing them to accumulate in the somatic follicle cells. This and other noncoding functions of the osk mRNA are mediated by multiple sequence elements with distinct roles. One role, provided by numerous binding sites in two distinct regions of the osk 3′ UTR, is to sequester the translational regulator Bruno (Bru), which itself controls translation of osk mRNA. This defines a novel regulatory circuit, with Bru restricting the activity of osk, and osk in turn restricting the activity of Bru. Other functional elements, which do not bind Bru and are positioned close to the 3′ end of the RNA, act in the oocyte and are essential. Despite the different roles played by the different types of elements contributing to RNA function, mutation of any leads to accumulation of the germline regulatory factors in the follicle cells.
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影响因子：
64.5
作者：
LEHMANN, R;NUSSLEINVOLHARD, C
通讯作者：
NUSSLEINVOLHARD, C
影响因子：
4.6
作者：
Jenny, Andreas;Hachet, Olivier;Ephrussi, Anne
通讯作者：
Ephrussi, Anne
DOI：
10.1073/pnas.0535280100
发表时间：
2003-03-04
影响因子：
11.1
作者：
Gong, WJ;Golic, KG
通讯作者：
Golic, KG
影响因子：
10.5
作者：
Ghabrial, A;Ray, RP;Schüpbach, T
通讯作者：
Schüpbach, T
影响因子：
3.6
作者：
Kloc, Malgorzata;Dallaire, Paul;Major, Francois
通讯作者：
Major, Francois