Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA.
Cloning and Characterization of TaTGW-7A Gene Associated with Grain Weight in Wheat via SLAF-seq-BSA.
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DOI：
10.3389/fpls.2016.01902
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发表时间：
2016
影响因子：
5.6
通讯作者：
Chang C
中科院分区：
文献类型：
--
作者：
Hu MJ;Zhang HP;Liu K;Cao JJ;Wang SX;Jiang H;Wu ZY;Lu J;Zhu XF;Xia XC;Sun GL;Ma CX;Chang C
小麦（Triticum aestivum L.）的千粒重（TGW）对籽粒产量有显著贡献。在本研究中，通过对京411和红芒春21杂交衍生的重组自交系（RIL）DNA混合池进行特异位点扩增片段测序（SLAF - seq），鉴定出一个与千粒重相关的候选基因。该基因位于7A染色体上，被命名为TaTGW - 7A，具有完整的基因组序列和一个开放阅读框（ORF）。在TaTGW - 7A位点的两个等位基因之间的第一个外显子中存在一个单核苷酸多态性（SNP），导致缬氨酸（Val）被丙氨酸（Ala）取代，对应着千粒重从高到低的变化。开发了酶切扩增多态性序列（CAPS）（TGW7A）和插入缺失（InDel）（TG9）标记，分别用于区分对应高千粒重和低千粒重的两个等位基因TaTGW - 7Aa和TaTGW - 7Ab。一个与TaTGW - 7A共分离的主效数量性状位点（QTL）在五个环境下的重组自交系群体中解释了21.7% - 27.1%的千粒重表型变异。TaTGW - 7A与千粒重的关联在一个自然群体和中国微核心种质中得到进一步验证。实时定量PCR显示，在籽粒发育过程中，TaTGW - 7Aa的转录水平高于TaTGW - 7Ab。在中国微核心种质（65.0%）和501个小麦品种（86.0%）中，高千粒重的优势等位基因TaTGW - 7Aa的高频出现表明该等位基因在小麦育种中受到强烈的正向选择。分子标记TGW7A和TG9可用于育种计划中千粒重的改良。
Thousand-grain weight (TGW) of wheat (Triticum aestivum L.) contributes significantly to grain yield. In the present study, a candidate gene associated with TGW was identified through specific-locus amplified fragment sequencing (SLAF-seq) of DNA bulks of recombinant inbred lines (RIL) derived from the cross between Jing 411 and Hongmangchun 21. The gene was located on chromosome 7A, designated as TaTGW-7A with a complete genome sequence and an open reading frame (ORF). A single nucleotide polymorphism (SNP) was present in the first exon between two alleles at TaTGW-7A locus, resulting in a Val to Ala substitution, corresponding to a change from higher to lower TGW. Cleaved amplified polymorphic sequence (CAPS) (TGW7A) and InDel (TG9) markers were developed to discriminate the two alleles TaTGW-7Aa and TaTGW-7Ab for higher and lower TGW, respectively. A major QTL co-segregating with TaTGW-7A explained 21.7–27.1% of phenotypic variance for TGW in the RIL population across five environments. The association of TaTGW-7A with TGW was further validated in a natural population and Chinese mini-core collections. Quantitative real-time PCR revealed higher transcript levels of TaTGW-7Aa than those of TaTGW-7Ab during grain development. High frequencies of the superior allele TaTGW-7Aa for higher TGW in Chinese mini-core collections (65.0%) and 501 wheat varieties (86.0%) indicated a strong and positive selection of this allele in wheat breeding. The molecular markers TGW7A and TG9 can be used for improvement of TGW in breeding programs.
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影响因子：
3.3
作者：
Breseghello, F;Sorrells, ME
通讯作者：
Sorrells, ME
DOI：
10.1073/pnas.0402251101
发表时间：
2004-10-26
影响因子：
11.1
作者：
Choi, HK;Mun, JH;Cook, DR
通讯作者：
Cook, DR
影响因子：
14.9
作者：
Harris, MA;Clark, J;White, R
通讯作者：
White, R
影响因子：
5.4
作者：
Huang, X. Q.;Cloutier, S.;Brown, P. D.
通讯作者：
Brown, P. D.
影响因子：
2.1
作者：
Kang, HW;Cho, YG;Eun, MY
通讯作者：
Eun, MY