Nerve-induced remodeling of muscle basal lamina during synaptogenesis.
Nerve-induced remodeling of muscle basal lamina during synaptogenesis.
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DOI：
10.1083/jcb.102.3.863
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发表时间：
1986-03
影响因子：
7.8
通讯作者：
ANDERSON, MJ
中科院分区：
文献类型：
--
作者：
ANDERSON, MJ
为了确定突触发生过程中连接基板沉积的调控机制，我们对非洲爪蟾胚胎培养的神经细胞和肌肉细胞进行了免疫细胞化学实验。在一些实验中，用基层蛋白多糖特异性荧光单克隆抗体脉冲标记后，对单个肌肉细胞进行了连续观察。在其他研究中，旧的和新的蛋白多糖分子被不同地标记为与对比荧光染料偶联的抗体。这些观察结果表明，抗体标记的蛋白聚糖表面沉积物在发育中的肌肉细胞上保持形态学稳定数天。然而，在同一时期，蛋白多糖积累的新位点主要包含最近暴露在细胞表面的抗原位点。当肌肉细胞受到胆碱能神经突的支配时，新的蛋白多糖在发育中的神经肌肉连接处被诱导积累，这些也几乎完全由最近沉积的抗原组成。在较老的肌肉培养中，许多细胞在其表面具有相对较高的背景抗原浓度，发育中的神经肌肉连接最初显示出与相邻的连接外肌肉表面相比，蛋白聚糖位点密度显着降低。在突触发育的后期，大部分神经周围区域最终充满了密集的神经诱导的蛋白多糖斑块。因此，运动神经元似乎对肌肉基板组织有两种表面上矛盾的作用。它们首先导致任何存在的连接外蛋白多糖从细胞接触的途径中移除，然后诱导在发育中的连接基板内沉积最近合成的蛋白多糖的致密斑块。这一观察结果表明，已经涉及组织重塑的蛋白水解酶系统也可能在突触发生过程中参与神经和肌肉细胞之间的诱导相互作用。
To identify mechanisms that regulate the deposition of the junctional basal lamina during synaptogenesis, immunocytochemical experiments were carried out on cultured nerve and muscle cells derived from Xenopus laevis embryos. In some experiments successive observations were made on individual muscle cells after pulse-labeling with a fluorescent monoclonal antibody specific for a basal lamina proteoglycan. In others, old and new proteoglycan molecules were differentially labeled with antibody conjugated to contrasting fluorochromes. These observations revealed that surface deposits of antibody-labeled proteoglycan remain morphologically stable for several days on developing muscle cells. Over the same period, however, new sites of proteoglycan accumulation formed that contained primarily those antigenic sites recently exposed at the cell surface. When muscle cells became innervated by cholinergic neurites, new proteoglycan accumulations were induced at the developing neuromuscular junctions, and these too were composed almost exclusively of recently deposited antigen. In older muscle cultures, where many cells possessed relatively high background concentrations of antigen over their surfaces, developing neuromuscular junctions initially showed a markedly reduced proteoglycan site-density compared with the adjacent, extrajunctional muscle surface. Much of this perineural region eventually became filled with dense, nerve induced proteoglycan plaques at later stages of synapse development. Motoneurons thus appear to have two, superficially paradoxical effects on muscle basal lamina organization. They first cause the removal of any existing, extrajunctional proteoglycan from the path of cell contact, and then induce the deposition of dense plaques of recently synthesized proteoglycan within the developing junctional basal lamina. This observation suggests that the proteolytic enzyme systems that have already been implicated in tissue remodeling may also contribute to the inductive interaction between nerve and muscle cells during synaptogenesis.
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DOI：
10.1083/jcb.99.5.1769
发表时间：
1984-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Anderson MJ;Klier FG;Tanguay KE
通讯作者：
Tanguay KE
影响因子：
5.5
作者：
ANDERSON, MJ;COHEN, MW
通讯作者：
COHEN, MW
影响因子：
7.8
作者：
BLOCH, RJ;HALL, ZW
通讯作者：
HALL, ZW
DOI：
10.1073/pnas.78.8.5230
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FROEHNER, SC;GULBRANDSEN, V;COHEN, JB
通讯作者：
COHEN, JB
DOI：
10.1083/jcb.99.2.615
发表时间：
1984-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Godfrey EW;Nitkin RM;Wallace BG;Rubin LL;McMahan UJ
通讯作者：
McMahan UJ