Acetylcholine receptor aggregation parallels the deposition of a basal lamina proteoglycan during development of the neuromuscular junction.
Acetylcholine receptor aggregation parallels the deposition of a basal lamina proteoglycan during development of the neuromuscular junction.
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DOI：
10.1083/jcb.99.5.1769
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发表时间：
1984-11
期刊：
影响因子：
--
通讯作者：
Tanguay KE
中科院分区：
文献类型：
--
作者：
Anderson MJ;Klier FG;Tanguay KE
为了确定突触分化的时间过程，我们对培养中发育的神经接触肌细胞进行了连续观察。培养物用荧光α-银环蛇毒素染色，或在荧光单克隆抗体存在下维持。这些探针是针对乙酰胆碱受体（AChR）和基底膜蛋白聚糖，在成人神经肌肉接头显示几乎一致的表面组织的物质。在其他实验中，在AChR积累的不同阶段选择培养中发育的单个肌肉细胞，并在电子显微镜下观察沿着神经-肌肉接触的整个路径连续切片。结果表明，神经诱导的AChR聚集体和相邻的蛋白多糖斑块的形成是紧密耦合的突触形成的整个早期阶段。AChR和蛋白聚糖的交界处积累的发展出现和增长逐步，整个神经周围区，延伸沿着肌肉表面上的神经过程的两侧几个微米。不像交界处AChR积累，在一天内消失的去神经，交界处和交界外蛋白多糖存款的大小和形态稳定。连接蛋白多糖沉积物似乎对应于基底层的离散超微结构斑块，其最初由无板层的肌肉表面的广泛扩张分离。在AChR和蛋白多糖沿着神经接触路径积累的过程中，这一基底层的程度和相应的突触后膜增厚也增加。突触前分化的突触囊泡簇成为可检测的神经肌肉接头后，形成的突触后斑块含有乙酰胆碱受体和蛋白多糖。它的结论是，运动神经诱导逐渐形成和增长的乙酰胆碱受体聚集体和稳定的基底层蛋白多糖沉积在肌肉表面的神经肌肉接头的发展过程中。
To determine the time course of synaptic differentiation, we made successive observations on identified, nerve-contacted muscle cells developing in culture. The cultures had either been stained with fluorescent alpha-bungarotoxin, or were maintained in the presence of a fluorescent monoclonal antibody. These probes are directed at acetylcholine receptors (AChR) and a basal lamina proteoglycan, substances that show nearly congruent surface organizations at the adult neuromuscular junction. In other experiments individual muscle cells developing in culture were selected at different stages of AChR accumulation and examined in the electron microscope after serial sectioning along the entire path of nerve-muscle contact. The results indicate that the nerve-induced formation of AChR aggregates and adjacent plaques of proteoglycan is closely coupled throughout early stages of synapse formation. Developing junctional accumulations of AChR and proteoglycan appeared and grew progressively, throughout a perineural zone that extended along the muscle surface for several micrometers on either side of the nerve process. Unlike junctional AChR accumulations, which disappeared within a day of denervation, both junctional and extrajunctional proteoglycan deposits were stable in size and morphology. Junctional proteoglycan deposits appeared to correspond to discrete ultrastructural plaques of basal lamina, which were initially separated by broad expanses of lamina-free muscle surface. The extent of this basal lamina, and a corresponding thickening of the postsynaptic membrane, also increased during the accumulation of AChR and proteoglycan along the path of nerve contact. Presynaptic differentiation of synaptic vesicle clusters became detectable at the developing neuromuscular junction only after the formation of postsynaptic plaques containing both AChR and proteoglycan. It is concluded that motor nerves induce a gradual formation and growth of AChR aggregates and stable basal lamina proteoglycan deposits on the muscle surface during development of the neuromuscular junction.
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DOI：
10.1073/pnas.73.12.4594
发表时间：
1976-01-01
影响因子：
11.1
作者：
AXELROD, D;RAVDIN, P;PODLESKI, TR
通讯作者：
PODLESKI, TR
DOI：
10.1007/bf01098386
发表时间：
1974-01-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
BIRKS, RI
通讯作者：
BIRKS, RI
影响因子：
5.5
作者：
LOMO, T;SLATER, CR
通讯作者：
SLATER, CR
DOI：
10.1098/rspb.1966.0008
发表时间：
1966-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BARKER, D;IP, MC
通讯作者：
IP, MC
影响因子：
5.5
作者：
ANDERSON, MJ;COHEN, MW
通讯作者：
COHEN, MW