Single-Cell RNA Sequencing of Human, Macaque, and Mouse Testes Uncovers Conserved and Divergent Features of Mammalian Spermatogenesis.
Single-Cell RNA Sequencing of Human, Macaque, and Mouse Testes Uncovers Conserved and Divergent Features of Mammalian Spermatogenesis.
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DOI：
10.1016/j.devcel.2020.05.010
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发表时间：
2020-08-24
影响因子：
11.8
通讯作者：
Hammoud SS
中科院分区：
文献类型：
--
作者：
Shami AN;Zheng X;Munyoki SK;Ma Q;Manske GL;Green CD;Sukhwani M;Orwig KE;Li JZ;Hammoud SS
精子发生是一个高度受调控的过程，它产生精子，将遗传信息传递给下一代。虽然在老鼠身上进行了广泛的研究，但我们目前对灵长类精子发生的了解仅限于由啮齿动物数据中的状态特定标记定义的种群。由于这一过程在持续时间和分化层次上存在物种间差异，目前尚不清楚分子标记和细胞状态是如何保守的，或者是如何从小鼠分化到人类的。为了解决这一挑战，我们使用单细胞RNA测序来识别人类、猕猴和小鼠睾丸的主要生殖细胞和体细胞类型的转录特征。这种方法揭示了精子发生过程中表达的相似性和差异性，包括精原细胞的干祖库、分化标记、减数分裂的潜在调节因子、精子细胞分化过程中的RNA周转以及生殖细胞与胞体的通讯。这些数据集为未来灵长类生殖细胞发育和体外配子发生的有针对性的机制研究提供了丰富的基础。
Spermatogenesis is a highly regulated process that produces sperm to transmit genetic information to the next generation. Although extensively studied in mice, our current understanding of primate spermatogenesis is limited to populations defined by state-specific markers from rodent data. As between-species differences have been reported in the duration and differentiation hierarchy of this process, it remains unclear how molecular markers and cell states are conserved or have diverged from mice to man. To address this challenge, we employ single-cell RNA-sequencing to identify transcriptional signatures of major germ and somatic cell-types of the testes in human, macaque, and mice. This approach reveals similarities and differences in expression throughout spermatogenesis, including the stem/progenitor pool of spermatogonia, markers of differentiation, potential regulators of meiosis, RNA turnover during spermatid differentiation, and germ cell-soma communication. These datasets provide a rich foundation for future targeted mechanistic studies of primate germ cell development and in vitro gametogenesis.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
ANTAR, M
影响因子：
3.6
作者：
DEROOIJ, DG;VANALPHEN, MMA;VANDEKANT, HJG
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