Overexpression of DoBAM1 from Yam (Dioscorea opposita Thunb.) Enhances Cold Tolerance in Transgenic Tobacco.
Overexpression of DoBAM1 from Yam (Dioscorea opposita Thunb.) Enhances Cold Tolerance in Transgenic Tobacco.
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山药 (Dioscorea opposita Thunb.) 中 DoBAM1 的过度表达增强了转基因烟草的耐冷性
作者：
β-淀粉酶在植物生长发育和对非生物胁迫的响应中起着重要作用。在本研究中，从山药中鉴定出5个DoBAM成员。从山药品种别克齐和大和昌玉中分离到一个新的β-淀粉酶基因BAM1，命名为DoBAM1。DoBAM1的开放阅读框(ORF)为2806bp，编码543个氨基酸。亚细胞定位分析表明，DoBAM1定位于细胞膜和细胞质。大和昌玉的淀粉含量、β-淀粉酶活性和DoBAM1的表达均高于碧客齐。烟草中DOBAM1的过表达促进了烟草体内可溶性糖和叶绿素含量的积累，提高了过氧化物酶、超氧化物歧化酶、过氧化氢酶和β-淀粉酶的活性。在低温处理下，我们观察到WT植株的DoBAM1表达上调，淀粉含量和丙二醛(MDA)积累量降低。综上所述，这些结果表明，DoBAM1的过度表达在耐冷性方面发挥了高级作用，至少部分是通过提高能够作为渗透调节剂或抗氧化剂的可溶性糖的水平。
β-amylase (BAM) plays an important role in plant development and response to abiotic stresses. In this study, 5 DoBAM members were identified in yam (Dioscorea opposita Thunb.). A novel β-amylase gene BAM1, (named DoBAM1), was isolated from yam varieties Bikeqi and Dahechangyu. The open reading frame (ORF) of DoBAM1 is 2806 bp and encodes 543 amino acids. Subcellular localization analysis indicates that DoBAM1 localizes to the cell membrane and cytoplasm. In the yam variety Dahechangyu, the starch content, β-amylase activity, and expression of DoBAM1 were characterized and found to all be higher than in Bikeqi. DoBAM1 overexpression in tobacco is shown to promote the accumulation of soluble sugar and chlorophyll content and to increase the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and β-amylase. Under cold treatment, we observed the induced upregulation of DoBAM1 and lower starch content and malondialdehyde (MDA) accumulation than in WT plants. In conclusion, these results demonstrate that DoBAM1 overexpression plays an advanced role in cold tolerance, at least in part by raising the levels of soluble sugars that are capable of acting as osmolytes or antioxidants.
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影响因子：
6.9
作者：
Krasensky J;Jonak C
通讯作者：
Jonak C
影响因子：
5.3
作者：
Liang G;He H;Nai G;Feng L;Li Y;Zhou Q;Ma Z;Yue Y;Chen B;Mao J
通讯作者：
Mao J
影响因子：
4.4
作者：
通讯作者：
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一氧化氮和赤霉素预浸种诱导小麦发芽过程中的耐冷性
影响因子：
4.2
作者：
Li, Xiangnan;Jiang, Haidong;Jiang, Dong
通讯作者：
Jiang, Dong
影响因子：
4.3
作者：
Li, Ning;Zhang, Shujuan;Zhang, Juren
通讯作者：
Zhang, Juren