Suppression of bactericidal activity of human polymorphonuclear leukocytes by Bacteroides gingivalis
Suppression of bactericidal activity of human polymorphonuclear leukocytes by Bacteroides gingivalis
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牙龈拟杆菌对人多形核白细胞杀菌活性的抑制
DOI：
10.1128/iai.58.2.406-411.1990
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发表时间：
1990
影响因子：
3.1
通讯作者：
M. Aono
中科院分区：
文献类型：
--
作者：
M. Yoneda;K. Maeda;M. Aono
本文研究了口腔微生物培养上清对人中性粒细胞（PMNs）杀菌活性的直接影响。通过计数存活细菌来检查用牙龈拟杆菌、中间拟杆菌、产黑色素拟杆菌或磷酸盐缓冲盐水的上清液预孵育的PMN的杀菌活性。B。发现牙龈炎处理的PMN在存在或不存在血清的情况下具有降低的杀死细菌的能力。用B培养上清预孵育的PMN的化学发光反应。与用磷酸盐缓冲盐水或其他细菌培养上清液预孵育的PMN相比，牙龈炎的PMN的粘附率显著降低。用B的培养上清预孵育后，无论是甲硫氨酰亮氨酰苯丙氨酸还是佛波醇肉豆蔻酸酯刺激的PMN产生超氧阴离子（O2-）的量均减少。牙龈炎然而，据观察，有更多的减少超氧阴离子（O2-）的生产刺激与甲酰甲硫氨酰亮氨酰苯丙氨酸相比，刺激佛波醇肉豆蔻酸乙酸酯。这些结果表明，B.牙龈炎释放一种因子，该因子通过调节活性氧物质的产生来干扰PMN的杀菌活性。对杀菌活性的这些抑制作用可能在该微生物的发病机制中是重要的。
The direct effects of the culture supernatant of oral microorganisms on the bactericidal activity of human polymorphonuclear leukocytes (PMNs) were investigated. The bactericidal activity of PMNs, which were preincubated with the supernatant of Bacteroides gingivalis, Bacteroides intermedius, Bacteroides melaninogenicus or phosphate-buffered saline, was examined by counting the surviving bacteria. B. gingivalis-treated PMNs were found to have a diminished ability for killing bacteria in the presence or absence of serum. The chemiluminescence response of PMNs, which were preincubated with the culture supernatant of B. gingivalis, was strikingly reduced compared with that of PMNs preincubated with phosphate-buffered saline or other bacterial culture supernatants. The production of superoxide anion (O2-) by PMNs stimulated with either formyl-methionyl-leucyl-phenylalanine or phorbol myristate acetate was reduced in both cases after the PMNs were preincubated with the culture supernatant of B. gingivalis. However, it was observed that there was more reduction in superoxide anion (O2-) production stimulated with formyl-methionyl-leucyl-phenylalanine compared with that stimulated with phorbol myristate acetate. These results suggest that B. gingivalis releases a factor which interferes with the bactericidal activity of PMNs by modulating the generation of reactive oxygen species. These suppressive effects on bactericidal activity may be important in the pathogenesis of this microorganism.
影响因子：
56.9
作者：
HOLT, SC;EBERSOLE, J;KORNMAN, KS
通讯作者：
KORNMAN, KS
影响因子：
6.7
作者：
DZINK, JL;SOCRANSKY, SS;HAFFAJEE, AD
通讯作者：
HAFFAJEE, AD
中性粒细胞中的信号转导和细胞骨架激活。
影响因子：
33.6
作者：
Omann,GM;Allen,RA;Bokoch,GM;Painter,RG;Traynor,AE;Sklar,LA
通讯作者：
Sklar,LA