A newly identified interaction between nucleolar NPM1/B23 and the HTLV-I basic leucine zipper factor in HTLV-1 infected cells.
A newly identified interaction between nucleolar NPM1/B23 and the HTLV-I basic leucine zipper factor in HTLV-1 infected cells.
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新发现的 HTLV-1 感染细胞中核仁 NPM1/B23 与 HTLV-I 碱性亮氨酸拉链因子之间的相互作用
DOI：
10.3389/fmicb.2022.988944
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
人类T细胞白血病病毒1型是HTLV-1相关脊髓病/热带痉挛性下肢轻瘫和成人T细胞白血病淋巴瘤（ATL）的病原体。HTLV-1碱性亮氨酸拉链因子（HBZ）与这种病毒的致癌特性有关，尽管确切的机制尚不清楚。在这项研究中，我们确定了核磷蛋白（NPM 1/B23）作为HBZ的一个新的相互作用伙伴。我们发现sHBZ和丰度较低的uHBZ亚型与感染细胞和HTLV-1阳性患者细胞中的核仁NPM1/B23相互作用，而不像相关的非致白血病HTLV-2，-3和-4病毒的等效反义蛋白。我们进一步证明sHBZ与NPM1/B23的结合对RNase敏感。有趣的是，sHBZ被证明与其自身的RNA相互作用。通过siRNA和过表达实验，我们进一步提供证据表明NPM1/B23对病毒基因表达具有负作用，对细胞转化具有潜在影响。因此，我们的研究结果提供了一个新的见解HBZ结合伙伴在细胞定位和细胞增殖的潜在功能，并应导致更好地了解HBZ和ATL发展之间的联系。
Human T-cell leukemia virus type 1 is the causative agent of HTLV-1-associated myelopathy/tropical spastic paraparesis and adult T-cell leukemia-lymphoma (ATL). The HTLV-1 basic leucine zipper factor (HBZ) has been associated to the cancer-inducing properties of this virus, although the exact mechanism is unknown. In this study, we identified nucleophosmin (NPM1/B23) as a new interaction partner of HBZ. We show that sHBZ and the less abundant uHBZ isoform interact with nucleolar NPM1/B23 in infected cells and HTLV-1 positive patient cells, unlike equivalent antisense proteins of related non-leukemogenic HTLV-2, −3 and-4 viruses. We further demonstrate that sHBZ association to NPM1/B23 is sensitive to RNase. Interestingly, sHBZ was shown to interact with its own RNA. Through siRNA and overexpression experiments, we further provide evidence that NPM1/B23 acts negatively on viral gene expression with potential impact on cell transformation. Our results hence provide a new insight over HBZ-binding partners in relation to cellular localization and potential function on cell proliferation and should lead to a better understanding of the link between HBZ and ATL development.
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影响因子：
3.2
作者：
BIGGIOGERA, M;FAKAN, S;BUSCH, H
通讯作者：
BUSCH, H
影响因子：
5.2
作者：
Forlani, Greta;Baratella, Marco;Accolla, Roberto S.
通讯作者：
Accolla, Roberto S.
影响因子：
1.5
作者：
Arizala JAC;Takahashi M;Burnett JC;Ouellet DL;Li H;Rossi JJ
通讯作者：
Rossi JJ
影响因子：
8.4
作者：
Gao, Hongzhi;Ye, Xiang;Kubota, Ryuji
通讯作者：
Kubota, Ryuji
影响因子：
20.3
作者：
Falini, Brunangelo;Martelli, Maria Paola;Mecucci, Cristina
通讯作者：
Mecucci, Cristina