In vitro articular cartilage growth with sequential application of IGF-1 and TGF-β1 enhances volumetric growth and maintains compressive properties.
In vitro articular cartilage growth with sequential application of IGF-1 and TGF-β1 enhances volumetric growth and maintains compressive properties.
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DOI：
10.1115/1.4005851
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发表时间：
2012-03
期刊：
影响因子：
--
通讯作者：
Klisch SM
中科院分区：
文献类型：
--
作者：
Balcom NT;Berg-Johansen B;Dills KJ;Van Donk JR;Williams GM;Chen AC;Hazelwood SJ;Sah RL;Klisch SM
在体外培养与胰岛素样生长因子-1（IGF-1）和转化生长因子-β 1（TGF-β 1）先前已被证明差异调节未成熟的牛关节软骨的生长。IGF-1刺激扩张性生长，但降低压缩模量并增加压缩泊松比，而TGF-β 1保持组织尺寸，增加压缩模量并降低压缩泊松比。本研究的假设是，在体外培养过程中连续应用IGF-1和TGF-β 1产生的几何和压缩力学性能介于单独使用任一生长因子时产生的极值之间。收获未成熟的牛关节软骨样本，并且或者未处理（D0，即第0天），或者在体外用IGF-1培养6天（D6 IGF），用IGF-1培养12天（D12 IGF），或者用IGF-1培养6天，然后用TGF-β 1培养6天（D12 SEQ，即顺序）。治疗后，所有标本进行了测试的几何，生物化学和压缩机械性能。相对于D0，D12 SEQ处理增强了体积生长，但其值低于D12 IGF。此外，D12 SEQ处理将压缩模量和泊松比分别保持在比D12 IGF更高和更低的值。考虑到先前描述的单独用TGF-β 1处理12天的效果，D12 SEQ诱导的生长和机械性质变化在单独用IGF-1或TGF-β 1产生的那些之间。结果表明，在临床修复策略中，改变选择的生长因子（包括IGF-1和TGF-β 1）的持续时间，以更精确地调节未成熟软骨移植组织的几何、生物化学和机械特性是可能的。
In vitro cultures with insulin-like growth factor–1 (IGF-1) and transforming growth factor–beta 1 (TGF-β1) have previously been shown to differentially modulate the growth of immature bovine articular cartilage. IGF-1 stimulates expansive growth yet decreases compressive moduli and increases compressive Poisson’s ratios, whereas TGF- β1 maintains tissue size, increases compressive moduli, and decreases compressive Poisson’s ratios. The current study’s hypothesis was that sequential application of IGF-1 and TGF- β1 during in vitro culture produces geometric and compressive mechanical properties that lie between extreme values produced when using either growth factor alone. Immature bovine articular cartilage specimens were harvested and either untreated (D0 i.e. day zero) or cultured in vitro for either six days with IGF-1 (D6 IGF), 12 days with IGF-1 (D12 IGF), or six days with IGF-1 followed by six days with TGF-β1 (D12 SEQ i.e. sequential). Following treatment, all specimens were tested for geometric, biochemical, and compressive mechanical properties. Relative to D0, D12 SEQ treatment enhanced volumetric growth but to a lower value than that for D12 IGF. Furthermore, D12 SEQ treatment maintained compressive moduli and Poisson’s ratios at values higher and lower, respectively, than those for D12 IGF. Considering the previously described effects of 12 days of treatment with TGF-β1 alone, D12 SEQ induced both growth and mechanical property changes between those produced with either IGF-1 or TGF-β1 alone. The results suggest that it may be possible to vary the durations of select growth factors, including IGF-1 and TGF-β1, to more precisely modulate the geometric, biochemical, and mechanical properties of immature cartilage graft tissue in clinical repair strategies.
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影响因子：
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