Phytophthora palmivora establishes tissue-specific intracellular infection structures in the earliest divergent land plant lineage.
Phytophthora palmivora establishes tissue-specific intracellular infection structures in the earliest divergent land plant lineage.
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DOI：
10.1073/pnas.1717900115
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发表时间：
2018-04-17
影响因子：
11.1
通讯作者：
Schornack S
中科院分区：
文献类型：
--
作者：
Carella P;Gogleva A;Tomaselli M;Alfs C;Schornack S
尽管地钱作为最早的分歧陆地植物谱系支持真菌共生的重要性，它是未知的丝状病原体是否可以建立这些非维管植物的活细胞内的细胞内相互作用。在这里，我们证明了卵菌病原体入侵地钱和相关的苔类植物，形成细胞内的光合层细胞内的感染结构。缺乏这一组织层的植物表现出增强的抗感染能力，揭示了复杂的叶状体苔类植物的结构易感因素。此外，我们发现，专用的宿主细胞运输蛋白被招募到苔类细胞内的病原体界面，支持细胞内对微生物入侵的反应起源于非维管植物的想法。植物在陆地上的扩张是一个形成性事件，给地球的地球化学和生物群带来了深刻的变化。丝状真核微生物在陆地植物进化的早期就发展出了在植物组织中定居的能力，这一点在4亿年前的化石中得到了证实。然而，丝状微生物与早期分歧的陆地植物建立致病相互作用的程度尚不清楚。在这里，我们证明了广泛的宿主范围卵菌病原体棕榈疫霉殖民苔类，最早的分歧陆地植物谱系。我们发现，棕榈假单胞菌建立了一个复杂的组织特异性的相互作用地钱多形物，在那里它完成了一个完整的感染周期内的气室的背光合层。值得注意的是，棕榈疫霉内陷M.多形藻细胞具有吸器样结构，其积累宿主细胞运输机器和膜突触融合蛋白MpSYP13 B，但不积累相关的MpSYP13 A。我们的研究结果表明，丝状微生物的细胞内适应是一种古老的植物性状，被棕榈疫霉等病原体成功利用。
Despite the importance of liverworts as the earliest diverging land plant lineage to support fungal symbiosis, it is unknown whether filamentous pathogens can establish intracellular interactions within living cells of these nonvascular plants. Here, we demonstrate that an oomycete pathogen invades Marchantia polymorpha and related liverworts to form intracellular infection structures inside cells of the photosynthetic layer. Plants lacking this tissue layer display enhanced resistance to infection, revealing an architectural susceptibility factor in complex thalloid liverworts. Moreover, we show that dedicated host cellular trafficking proteins are recruited to pathogen interfaces within liverwort cells, supporting the idea that intracellular responses to microbial invasion originated in nonvascular plants. The expansion of plants onto land was a formative event that brought forth profound changes to the earth’s geochemistry and biota. Filamentous eukaryotic microbes developed the ability to colonize plant tissues early during the evolution of land plants, as demonstrated by intimate, symbiosis-like associations in >400 million-year-old fossils. However, the degree to which filamentous microbes establish pathogenic interactions with early divergent land plants is unclear. Here, we demonstrate that the broad host-range oomycete pathogen Phytophthora palmivora colonizes liverworts, the earliest divergent land plant lineage. We show that P. palmivora establishes a complex tissue-specific interaction with Marchantia polymorpha, where it completes a full infection cycle within air chambers of the dorsal photosynthetic layer. Remarkably, P. palmivora invaginates M. polymorpha cells with haustoria-like structures that accumulate host cellular trafficking machinery and the membrane syntaxin MpSYP13B, but not the related MpSYP13A. Our results indicate that the intracellular accommodation of filamentous microbes is an ancient plant trait that is successfully exploited by pathogens like P. palmivora.
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影响因子：
3.7
作者：
Ishizaki K;Nishihama R;Ueda M;Inoue K;Ishida S;Nishimura Y;Shikanai T;Kohchi T
通讯作者：
Kohchi T
影响因子：
4.5
作者：
Bozkurt, Tolga O.;Belhaj, Khaoula;Kamoun, Sophien
通讯作者：
Kamoun, Sophien
影响因子：
5.6
作者：
Ponce de León I;Montesano M
通讯作者：
Montesano M
影响因子：
4.9
作者：
Carella P;Schornack S
通讯作者：
Schornack S
影响因子：
4.2
作者：
Field, Katie J.;Duckett, Jeffrey G.;Pressel, Silvia
通讯作者：
Pressel, Silvia