Nondestructive assessment of sGAG content and distribution in normal and degraded rat articular cartilage via EPIC-microCT.
Nondestructive assessment of sGAG content and distribution in normal and degraded rat articular cartilage via EPIC-microCT.
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DOI：
10.1016/j.joca.2009.07.014
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发表时间：
2010-01
影响因子：
7
通讯作者：
Levenston, M. E.
中科院分区：
文献类型：
--
作者：
Xie, L.;Lin, A. S. P.;Guldberg, R. E.;Levenston, M. E.
The objective of this study was to evaluate the feasibility of quantifying the Equilibrium Partitioning of an Ionic Contrast agent via μCT (EPIC-μCT) to nondestructively assess sulfated glycosaminoglycan (sGAG) content and distribution in rat articular cartilage ex vivo, and in doing so to establish a paradigm for extension of this technique to other small animal models. After determination of an appropriate incubation time for the anionic contrast agent, EPIC-μCT was used to examine age-related differences in cartilage sGAG content between 4-, 8-, and 16-week old (n=5 each) male Wistar rats and to evaluate sGAG depletion in the right femora of each age group after 60 minutes of digestion with chondroitinase ABC. The EPIC-μCT measurements were validated by histological safranin-O staining, and reproducibility was evaluated by triplicate scans of six femora. Cartilage attenuation gradually increased with cumulative digestion time and reached a plateau at approximately 60 minutes with a 16.0% temporal increase (p<0.01). Average femoral articular cartilage attenuation increased by 14.2% from 4 to 8 weeks of age (p<0.01) and further increased by 2.5% from 8 to 16 weeks (p<0.05). After 60 minutes of digestion, femoral articular cartilage attenuations increased by 15-17% in each age group (p<0.01). Correspondingly, sGAG optical density decreased with age and digestion, and showed a linear correlation (r=−0.88, slope=−1.26, p<0.01, n=30) with EPIC-μCT cartilage attenuation. High reproducibility was indicated by a low coefficient of variation (1.5%) in cartilage attenuation. EPIC-μCT imaging provides high spatial resolution and sensitivity to assess sGAG content and three-dimensional distribution in rat femoral articular cartilage.
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影响因子：
2.8
作者：
Gray, Martha L.;Burstein, Deborah;Maroudas, Alice
通讯作者：
Maroudas, Alice
影响因子：
3.3
作者：
Gillis, A;Gray, M;Burstein, D
通讯作者：
Burstein, D
影响因子：
2.4
作者：
Laasanen, MS;Saarakkala, S;Jurvelin, JS
通讯作者：
Jurvelin, JS
影响因子：
3.8
作者：
Martin, I;Obradovic, B;Vunjak-Novakovic, G
通讯作者：
Vunjak-Novakovic, G
DOI：
10.1007/bf02331378
发表时间：
1996-08-01
期刊：
HISTOCHEMICAL JOURNAL
影响因子：
--
作者：
Kiraly, K;Lapvetelainen, T;Helminen, HJ
通讯作者：
Helminen, HJ