Loss of a conserved MAPK causes catastrophic failure in assembly of a specialized cilium-like structure in Toxoplasma gondii
Loss of a conserved MAPK causes catastrophic failure in assembly of a specialized cilium-like structure in Toxoplasma gondii
复制标题
弓形虫中保守 MAPK 的丢失会导致特殊纤毛样结构组装的灾难性失败
DOI：
10.1091/mbc.e19-11-0607
复制
发表时间：
2020
影响因子：
3.3
通讯作者：
Reese, Michael L.
中科院分区：
文献类型：
--
作者：
O’Shaughnessy, William J.;Hu, Xiaoyu;Beraki, Tsebaot;McDougal, Matthew;Reese, Michael L.
初级纤毛是控制多种细胞过程的重要组织中心。顶复门寄生虫，如刚地弓形虫，有一个特殊的纤毛样结构，称为锥状体，组织分泌和入侵机制，对寄生虫的生活方式至关重要。启动这种结构的生物起源的蛋白质在很大程度上是未知的。我们鉴定了保守激酶ERK 7在弓形虫中的直向同源物，认为它是锥体装配所必需的。ERK7已经耗尽的寄生虫在成熟后期失去它们的锥体，并且是不动的，因此不能侵入新的宿主细胞。这是迄今为止报道的最严重的圆锥体生物发生表型，并且由于ERK 7不是圆锥体蛋白的事实而更加引人注目，因为它仅定位于结构的基底。ERK 7最近被牵连在后生动物细胞中的纤毛发生，我们的数据表明，这种激酶在整个真核生物中调节纤毛发生有一个古老的和核心的作用。
Primary cilia are important organizing centers that control diverse cellular processes. Apicomplexan parasites likeToxoplasma gondiihave a specialized cilium-like structure called the conoid that organizes the secretory and invasion machinery critical for the parasites’ lifestyle. The proteins that initiate the biogenesis of this structure are largely unknown. We identified theToxoplasmaorthologue of the conserved kinase ERK7 as essential to conoid assembly. Parasites in which ERK7 has been depleted lose their conoids late during maturation and are immotile and thus unable to invade new host cells. This is the most severe phenotype to conoid biogenesis yet reported, and is made more striking by the fact that ERK7 is not a conoid protein, as it localizes just basal to the structure. ERK7 has been recently implicated in ciliogenesis in metazoan cells, and our data suggest that this kinase has an ancient and central role in regulating ciliogenesis throughout Eukaryota.
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影响因子：
4.6
作者：
Lévêque MF;Berry L;Besteiro S
通讯作者：
Besteiro S
DOI：
10.1016/b978-0-12-394309-5.00001-8
发表时间：
2012
影响因子：
--
作者：
Anderson-White, Brooke;Beck, Josh R.;Chen, Chun-Ti;Meissner, Markus;Bradley, Peter J.;Gubbels, Marc-Jan
通讯作者：
Gubbels, Marc-Jan
影响因子：
7.8
作者：
P. K. Hepler;C. G. Huff;H. Sprinz
通讯作者：
H. Sprinz
影响因子：
4.5
作者：
Lentini, Gaelle;Dubois, David J.;Frenal, Karine
通讯作者：
Frenal, Karine
影响因子：
3.7
作者：
Okamoto N;Keeling PJ
通讯作者：
Keeling PJ