Charge effect on the photoinactivation of Gram-negative and Gram-positive bacteria by cationic meso-substituted porphyrins.
Charge effect on the photoinactivation of Gram-negative and Gram-positive bacteria by cationic meso-substituted porphyrins.
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通过阳离子中二取代的卟啉对革兰氏阴性和革兰氏阳性细菌的光活化的电荷效应。
DOI：
10.1186/1471-2180-9-70
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发表时间：
2009-04-15
期刊：
影响因子：
4.2
通讯作者：
Almeida A
中科院分区：
文献类型：
--
作者：
Alves E;Costa L;Carvalho CM;Tomé JP;Faustino MA;Neves MG;Tomé AC;Cavaleiro JA;Cunha A;Almeida A
近年来，光动力抗微生物疗法已被用于使用阳离子卟啉作为光敏剂来有效地破坏革兰氏（+）和革兰氏（-）细菌。有越来越多的兴趣在这种方法中，即在寻找具有足够的结构特征的光敏剂的有效的光灭活过程。在这项研究中，我们提出了比较的效率不同的中位取代基，电荷数和电荷分布的七个阳离子卟啉，对革兰氏（+）细菌（粪肠球菌）和革兰氏（-）细菌（大肠杆菌）的光动力灭活。本研究补充了我们以前的工作，寻找光敏剂，可能被认为是很好的候选人的大范围的环境微生物的光灭活。将用不同光敏剂浓度（0.5、1.0和5.0 μ M）处理的细菌悬液（107 CFU mL-1）暴露于白色光（40 W m-2），总光剂量为64.8 J cm-2。对两种细菌菌株最有效的光敏剂是5.0 μ M的Tri-Py +-Me-PF和Tri-Py +-Me-CO2Me，光通量为64.8 J cm-2，导致> 7.0 log（> 99，999%）的光灭活。四价阳离子卟啉也被证明是一个很好的光敏剂对这两种细菌菌株。双阳离子和单阳离子卟啉是最有效的。卟啉结构中的正电荷数、电荷分布以及中位取代基对两种细菌的光失活有不同的影响。由于Tri-Py +-Me-PF卟啉使用较低的光剂量提供最高的对数减少，因此该光敏剂可以有效地光敏化大范围的环境细菌。两种细菌菌株在低光通量（40 W m-2）下的完全灭活意味着光动力学方法可以在自然光条件下应用于废水处理，这使得该技术在光源方面便宜且可行。
In recent times photodynamic antimicrobial therapy has been used to efficiently destroy Gram (+) and Gram (-) bacteria using cationic porphyrins as photosensitizers. There is an increasing interest in this approach, namely in the search of photosensitizers with adequate structural features for an efficient photoinactivation process. In this study we propose to compare the efficiency of seven cationic porphyrins differing in meso-substituent groups, charge number and charge distribution, on the photodynamic inactivation of a Gram (+) bacterium (Enterococcus faecalis) and of a Gram (-) bacterium (Escherichia coli). The present study complements our previous work on the search for photosensitizers that might be considered good candidates for the photoinactivation of a large spectrum of environmental microorganisms. Bacterial suspension (107 CFU mL-1) treated with different photosensitizers concentrations (0.5, 1.0 and 5.0 μM) were exposed to white light (40 W m-2) for a total light dose of 64.8 J cm-2. The most effective photosensitizers against both bacterial strains were the Tri-Py+-Me-PF and Tri-Py+-Me-CO2Me at 5.0 μM with a light fluence of 64.8 J cm-2, leading to > 7.0 log (> 99,999%) of photoinactivation. The tetracationic porphyrin also proved to be a good photosensitizer against both bacterial strains. Both di-cationic and the monocationic porphyrins were the least effective ones. The number of positive charges, the charge distribution in the porphyrins' structure and the meso-substituent groups seem to have different effects on the photoinactivation of both bacteria. As the Tri-Py+-Me-PF porphyrin provides the highest log reduction using lower light doses, this photosensitizer can efficiently photoinactivate a large spectrum of environmental bacteria. The complete inactivation of both bacterial strains with low light fluence (40 W m-2) means that the photodynamic approach can be applied to wastewater treatment under natural light conditions which makes this technology cheap and feasible in terms of the light source.
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影响因子：
3.5
作者：
Caminos, Daniel A.;Durantini, Edgardo N.
通讯作者：
Durantini, Edgardo N.
影响因子：
3.2
作者：
DAHL, TA;MIDDEN, WR;HARTMAN, PE
通讯作者：
HARTMAN, PE
影响因子：
--
作者：
Jemli, M;Alouini, Z;Gueddari, M
通讯作者：
Gueddari, M
影响因子：
1.4
作者：
Maestrin, Ana Paula J.;Ribeiro, Anderson O.;Cavaleiro, José A. S.
通讯作者：
Cavaleiro, José A. S.
影响因子：
3.1
作者：
Caminos, DA;Spesia, MB;Durantini, EN
通讯作者：
Durantini, EN