Vesicular stomatitis virus replicon expressing the VP2 outer capsid protein of bluetongue virus serotype 8 induces complete protection of sheep against challenge infection.
Vesicular stomatitis virus replicon expressing the VP2 outer capsid protein of bluetongue virus serotype 8 induces complete protection of sheep against challenge infection.
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DOI：
10.1186/1297-9716-45-64
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发表时间：
2014-06-13
影响因子：
4.4
通讯作者：
Zimmer G
中科院分区：
文献类型：
--
作者：
Kochinger S;Renevey N;Hofmann MA;Zimmer G
蓝舌病毒 (BTV) 是一种节肢动物传播的病原体，可导致反刍动物致命的出血性疾病。不同的 BTV 血清型遍布世界许多温带和热带地区。 2006年，BTV血清型8（BTV-8）首次在中欧和北欧出现。尽管这次疫情最终通过灭活病毒疫苗得到控制，但疫情造成的重大经济损失不仅来自牲畜疾病，还来自贸易限制。迄今为止，允许对受感染和已接种动物进行简单血清学区分的 BTV 疫苗 (DIVA) 尚未被批准用于牲畜。在这项研究中，我们基于单周期水泡性口炎病毒 (VSV) 载体生成了重组 RNA 复制子颗粒。用表达 BTV-8 外衣壳 VP2 蛋白的感染性 VSV 复制子颗粒对绵羊进行免疫，导致诱导产生 BTV-8 血清型特异性中和抗体。在用强毒力 BTV-8 菌株攻击后，接种疫苗的动物既没有出现疾病迹象，也没有出现病毒血症。相比之下，用重组 VP5（BTV 的第二个外衣壳蛋白）对绵羊进行免疫接种并不能提供保护。使用 ELISA 检测针对 VP7 抗原的抗体，可以轻松区分感染者和接种疫苗的动物。这些数据表明，VSV 复制子颗粒可能代表一个安全有效的疫苗平台，可用于控制 BTV-8 或其他血清型未来的疫情爆发，特别是在以前的非流行地区，在这些地区，区分接种疫苗的动物和受感染的动物至关重要。
Bluetongue virus (BTV) is an arthropod-borne pathogen that causes an often fatal, hemorrhagic disease in ruminants. Different BTV serotypes occur throughout many temperate and tropical regions of the world. In 2006, BTV serotype 8 (BTV-8) emerged in Central and Northern Europe for the first time. Although this outbreak was eventually controlled using inactivated virus vaccines, the epidemic caused significant economic losses not only from the disease in livestock but also from trade restrictions. To date, BTV vaccines that allow simple serological discrimination of infected and vaccinated animals (DIVA) have not been approved for use in livestock. In this study, we generated recombinant RNA replicon particles based on single-cycle vesicular stomatitis virus (VSV) vectors. Immunization of sheep with infectious VSV replicon particles expressing the outer capsid VP2 protein of BTV-8 resulted in induction of BTV-8 serotype-specific neutralizing antibodies. After challenge with a virulent BTV-8 strain, the vaccinated animals neither developed signs of disease nor showed viremia. In contrast, immunization of sheep with recombinant VP5 - the second outer capsid protein of BTV - did not confer protection. Discrimination of infected from vaccinated animals was readily achieved using an ELISA for detection of antibodies against the VP7 antigen. These data indicate that VSV replicon particles potentially represent a safe and efficacious vaccine platform with which to control future outbreaks by BTV-8 or other serotypes, especially in previously non-endemic regions where discrimination between vaccinated and infected animals is crucial.
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影响因子：
3.8
作者：
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通讯作者：
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