Multi-omics Investigation of Freeze Tolerance in the Amur Sleeper, an Aquatic Ectothermic Vertebrate.
Multi-omics Investigation of Freeze Tolerance in the Amur Sleeper, an Aquatic Ectothermic Vertebrate.
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DOI：
10.1093/molbev/msad040
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发表时间：
2023-03-04
影响因子：
10.7
通讯作者：
Yang, Liandong
中科院分区：
文献类型：
--
作者：
Jiang, Haifeng;Lv, Wenqi;Wang, Ying;Qian, Yuting;Wang, Cheng;Sun, Ning;Fang, Chengchi;Irwin, David M.;Gan, Xiaoni;He, Shunping;Yang, Liandong
关键词：
耐寒性，即生物体在内部冰层形成中生存的能力，是生活在寒冷环境中的一些外温动物采用的一种引人注目的生存策略。然而，这种显著适应的遗传基础在很大程度上是未知的。阿穆尔睡鱼(Perccottus Glenii)是已知的唯一一种耐冻鱼类，可以在整个身体被冰封的情况下越冬。在这里，我们对阿穆尔睡鼠的染色体水平的基因组进行了测序，并进行了比较基因组学、转录组和代谢学分析，以研究它在冷冻中存活的策略。进化分析表明，阿穆尔睡鼠在大约1507万年前与其最接近的非耐寒近亲背道而驰，经历了高速的蛋白质进化。转录组和代谢组数据确定了与低代谢、细胞应激反应和冷冻和解冻相关的冷冻保护剂积累有关的基因和代谢物的协调和组织特异性调节。有几个基因显示了蛋白质序列进化加速或家庭规模扩大的证据，这是对冷冻诱导的胁迫的适应性反应。具体地说，与细胞骨架稳定性、低温保护剂合成、跨膜运输和神经保护适应相关的遗传变化被认为是帮助冷冻存活的潜在关键创新。我们的工作为揭示支持低温脊椎动物耐冻性的分子适应提供了宝贵的资源和机会。
Freeze tolerance, the ability of an organism to survive internal ice formation, is a striking survival strategy employed by some ectotherms living in cold environments. However, the genetic bases of this remarkable adaptation are largely unknown. The Amur sleeper (Perccottus glenii), the only known freeze-tolerant fish species, can overwinter with its entire body frozen in ice. Here, we sequenced the chromosome-level genome of the Amur sleeper and performed comparative genomic, transcriptomic, and metabolomic analyses to investigate its strategies for surviving freezing. Evolutionary analysis suggested that the Amur sleeper diverged from its closest non-cold-hardy relative about 15.07 million years ago and has experienced a high rate of protein evolution. Transcriptomic and metabolomic data identified a coordinated and tissue-specific regulation of genes and metabolites involved in hypometabolism, cellular stress response, and cryoprotectant accumulation involved in freezing and thawing. Several genes show evidence of accelerated protein sequence evolution or family size expansion were found as adaptive responses to freezing-induced stresses. Specifically, genetic changes associated with cytoskeleton stability, cryoprotectant synthesis, transmembrane transport, and neuroprotective adaptations were identified as potentially key innovations that aid in freezing survival. Our work provides valuable resources and opportunities to unveil the molecular adaptations supporting freeze tolerance in ectothermic vertebrates.
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DOI：
10.1038/nrm2867
发表时间：
2010-04
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
--
作者：
Gordon, R. Ya.;Santalova, I. M.;Khutsian, S. S.
通讯作者：
Khutsian, S. S.
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4
作者：
Baldassari, Sara;Licchetta, Laura;Pippucci, Tommaso
通讯作者：
Pippucci, Tommaso
影响因子：
3.3
作者：
Jia Y;Zheng J;Liu S;Li F;Chi M;Cheng S;Gu Z
通讯作者：
Gu Z