CsINV5, a tea vacuolar invertase gene enhances cold tolerance in transgenic Arabidopsis.
CsINV5, a tea vacuolar invertase gene enhances cold tolerance in transgenic Arabidopsis.
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茶液泡转化酶基因 CsINV5 增强转基因拟南芥的耐冷性
DOI：
10.1186/s12870-018-1456-5
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发表时间：
2018-10-11
影响因子：
5.3
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Qian W;Xiao B;Wang L;Hao X;Yue C;Cao H;Wang Y;Li N;Yu Y;Zeng J;Yang Y;Wang X
植物极性转化酶（VIN）是植物生长发育的重要调控因子，对干旱、低温等非生物胁迫具有响应作用。就目前所知，VIN基因在茶树中的功能研究还很少。因此，有必要开展这一领域的研究。在这里，我们确定了VIN基因，CsINV 5，这是诱导的冷驯化和糖处理的茶树。组织化学分析结果表明，CsINV 5基因5′侧翼区1154 bp的序列驱动了GUS基因在拟南芥根、茎、叶、花和角果不同发育阶段的表达。此外，启动子缺失分析结果显示，一个LTRE相关的基序（CCGAAA）和WBOXHVISO 1基序（TGACT）的启动子区域内的CsINV 5的核心顺式元件在低温和糖信号，分别响应。此外，在拟南芥中过表达CsINV 5通过葡萄糖介导的对生长素信号的影响促进主根和侧根伸长。通过生理和RNA-seq分析发现，CsINV 5基因的过表达主要通过提高葡萄糖和果糖的含量，增加相应的己糖/蔗糖比，以及调节渗透势的抗寒相关基因（P5 CS 1、P5 CS2、AtLEA 3、COR 413-PM 1和COR 15 B）的转录来提高转基因拟南芥的抗寒性。综合实验结果表明，超表达CsINV 5可能通过改变细胞内糖含量和渗透调节相关途径来增强植物的耐冷性。本文的在线版本（10.1186/s12870-018-1456-5）包含补充材料，可供授权用户使用。
Vacuolar invertases (VINs) have been reported to regulate plant growth and development and respond to abiotic stresses such as drought and cold. With our best knowledge, the functions of VIN genes little have been reported in tea plant (Camellia sinensis L.). Therefore, it is necessary to develop research in this field. Here, we identified a VIN gene, CsINV5, which was induced by cold acclimation and sugar treatments in the tea plant. Histochemical assays results showed that the 1154 bp 5′-flanking sequence of CsINV5 drove β-glucuronidase (GUS) gene expression in roots, stems, leaves, flowers and siliques of transgenic Arabidopsis during different developmental stages. Moreover, promoter deletion analysis results revealed that an LTRE-related motif (CCGAAA) and a WBOXHVISO1 motif (TGACT) within the promoter region of CsINV5 were the core cis-elements in response to low temperature and sugar signaling, respectively. In addition, overexpression of CsINV5 in Arabidopsis promoted taproot and lateral root elongation through glucose-mediated effects on auxin signaling. Based on physiological and RNA-seq analysis, we found that overexpression of CsINV5 improved cold tolerance in transgenic Arabidopsis mainly by increasing the contents of glucose and fructose, the corresponding ratio of hexose to sucrose, and the transcription of osmotic-stress-related genes (P5CS1, P5CS2, AtLEA3, COR413-PM1 and COR15B) to adjust its osmotic potential. Comprehensive experimental results suggest that overexpression of CsINV5 may enhance the cold tolerance of plant through the modification of cellular sugar compounds contents and osmotic regulation related pathways. The online version of this article (10.1186/s12870-018-1456-5) contains supplementary material, which is available to authorized users.
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影响因子：
7.4
作者：
Gilmour, SJ;Sebolt, AM;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
5.1
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