Nicotinamide Riboside Improves Stemness of Human Adipose-Derived Stem Cells and Inhibits Terminal Adipocyte Differentiation.
Nicotinamide Riboside Improves Stemness of Human Adipose-Derived Stem Cells and Inhibits Terminal Adipocyte Differentiation.
复制标题
烟酰胺核苷改善人脂肪干细胞的干性并抑制脂肪细胞的终末分化。
DOI：
10.3390/ph16081134
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发表时间：
2023-08-10
期刊：
影响因子：
4.6
通讯作者：
Ejaz, Asim
中科院分区：
文献类型：
--
作者：
Chinnapaka, Somaiah;Malekzadeh, Hamid;Tirmizi, Zayaan;Arellano, Jose A.;Ejaz, Asim
关键词：
脂肪组织作为多余脂肪的储存场所，保护其他器官免受脂毒性的不利影响，在维持代谢动态平衡方面起着至关重要的作用。然而，衰老过程伴随着脂肪的重新分布，其特征是胰岛素敏感型皮下脂肪储备量减少，胰岛素抵抗内脏脂肪储存量增加。这种与年龄相关的脂肪组织分布变化对新陈代谢健康有影响。脂肪干细胞(ASCs)在脂肪组织再生中起着至关重要的作用。然而，衰老对ASCs的干性和再生能力产生了负面影响。氧化应激和线粒体功能障碍相关的细胞损伤的积累导致了老年ASCs的干性下降。烟酰胺腺嘌呤二核苷酸(NAD+)是一种重要的代谢物，参与维持细胞的动态平衡和干性。随着年龄的增长，NAD+水平的失调与代谢紊乱和茎的丧失有关。本研究旨在探讨NAD+前体烟酰胺核苷(NR)对体外培养的人ASCs干性的影响。我们的研究结果表明，脂肪形成伴随着线粒体活性的增加和活性氧物种(ROS)的产生。然而，NR处理导致ASCs线粒体活性和ROS产生减少。此外，给予NR可提高ASCs的干性相关基因的表达，并减轻其向脂肪细胞分化的倾向。这些结果表明，NR治疗有望成为恢复老年ASCs干性的一种潜在策略。进一步的研究，包括使用动物模型和人体研究的体内评估，将有必要验证这些发现，并确定这种公认的药物在增强衰老干细胞干性方面的临床潜力。
Adipose tissue plays a crucial role in maintaining metabolic homeostasis by serving as a storage site for excess fat and protecting other organs from the detrimental effects of lipotoxicity. However, the aging process is accompanied by a redistribution of fat, characterized by a decrease in insulin-sensitive subcutaneous adipose depot and an increase in insulin-resistant visceral adipose depot. This age-related alteration in adipose tissue distribution has implications for metabolic health. Adipose-derived stem cells (ASCs) play a vital role in the regeneration of adipose tissue. However, aging negatively impacts the stemness and regenerative potential of ASCs. The accumulation of oxidative stress and mitochondrial dysfunction-associated cellular damage contributes to the decline in stemness observed in aged ASCs. Nicotinamide adenine dinucleotide (NAD+) is a crucial metabolite that is involved in maintaining cellular homeostasis and stemness. The dysregulation of NAD+ levels with age has been associated with metabolic disorders and the loss of stemness. In this study, we aimed to investigate the effects of nicotinamide riboside (NR), a precursor of NAD+, on the stemness of human ASCs in cell culture. Our findings reveal that adipogenesis is accompanied by an increase in mitochondrial activity and the production of reactive oxygen species (ROS). However, treatment with NR leads to a reduction in mitochondrial activity and ROS production in ASCs. Furthermore, NR administration improves the stemness-related genes expression in ASCs and mitigates their propensity for adipocyte differentiation. These results suggest that NR treatment holds promise as a potential strategy to rejuvenate the stemness of aged ASCs. Further investigations, including in vivo evaluations using animal models and human studies, will be necessary to validate these findings and establish the clinical potential of this well-established drug for enhancing the stemness of aged stem cells.
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影响因子：
13.6
作者：
通讯作者：
--
DOI：
10.18632/aging.101197
发表时间：
2017-03-17
期刊：
Aging
影响因子：
--
作者：
Ejaz A;Mattesich M;Zwerschke W
通讯作者：
Zwerschke W
影响因子：
7.7
作者：
DEFRONZO, RA
通讯作者：
DEFRONZO, RA
影响因子：
4.2
作者：
Klimova, Nina;Long, Aaron;Kristian, Tibor
通讯作者：
Kristian, Tibor
影响因子：
4.6
作者：
Bigarella, Carolina L.;Liang, Raymond;Ghaffari, Saghi
通讯作者：
Ghaffari, Saghi