QTL mapping of combining ability and heterosis of agronomic traits in rice backcross recombinant inbred lines and hybrid crosses.
QTL mapping of combining ability and heterosis of agronomic traits in rice backcross recombinant inbred lines and hybrid crosses.
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水稻回交重组自交系及杂种农艺性状配合力及杂种优势的QTL定位
DOI：
10.1371/journal.pone.0028463
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hu Z
中科院分区：
文献类型：
--
作者：
Qu Z;Li L;Luo J;Wang P;Yu S;Mou T;Zheng X;Hu Z
背景配合力效应是决定下一阶段育种计划的非常有效的遗传参数。虽然在杂交育种中，以配合力评价为基础的育种策略得到了广泛的应用，但对配合力的遗传基础却知之甚少。配合力是一个受多基因控制的复杂性状。随着分子标记技术的出现和发展，利用QTL分析评价优良杂交稻配合力和杂种优势的遗传基础已成为可能。方法/主要发现在本研究中，我们首先建立了一个QTL定位方法，用于解剖农艺性状的配合力和杂种优势。利用3个水稻测交群体和1个BCRIL群体，对10个农艺性状进行了生物统计学和QTL分析。大多数性状的一般配合力和特殊配合力均达到显著水平，表明加性效应和非加性效应对表达水平的影响都很重要。在这10个性状中，检测到大量与BCRIL本身性状和一般配合力相关的加性效应QTL，以及与特殊配合力和杂种优势相关的显性效应QTL。结论利用QTL定位方法可以分析农艺性状的配合力。农艺性状配合力的QTL所揭示的特征与BCRIL本身的众多农艺性状QTL所揭示的特征相似。每个性状的配合力都定位到多个QTL（本研究中为1-6个）。结果表明，部分QTL具有多效性或紧密连锁。本研究对影响配合力和杂种优势的QTL进行了定位，为深入剖析配合力的遗传基础提供了有价值的信息。
Background Combining ability effects are very effective genetic parameters in deciding the next phase of breeding programs. Although some breeding strategies on the basis of evaluating combining ability have been utilized extensively in hybrid breeding, little is known about the genetic basis of combining ability. Combining ability is a complex trait that is controlled by polygenes. With the advent and development of molecular markers, it is feasible to evaluate the genetic bases of combining ability and heterosis of elite rice hybrids through QTL analysis. Methodology/Principal Findings In the present study, we first developed a QTL-mapping method for dissecting combining ability and heterosis of agronomic traits. With three testcross populations and a BCRIL population in rice, biometric and QTL analyses were conducted for ten agronomic traits. The significance of general combining ability and special combining ability for most of the traits indicated the importance of both additive and non-additive effects on expression levels. A large number of additive effect QTLs associated with performance per se of BCRIL and general combining ability, and dominant effect QTLs associated with special combining ability and heterosis were identified for the ten traits. Conclusions/Significance The combining ability of agronomic traits could be analyzed by the QTL mapping method. The characteristics revealed by the QTLs for combining ability of agronomic traits were similar with those by multitudinous QTLs for agronomic traits with performance per se of BCRIL. Several QTLs (1–6 in this study) were identified for each trait for combining ability. It demonstrated that some of the QTLs were pleiotropic or linked tightly with each other. The identification of QTLs responsible for combining ability and heterosis in the present study provides valuable information for dissecting genetic basis of combining ability.
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影响因子：
5.4
作者：
Schrag, Tobias A.;Moehring, Jens;Frisch, Matthias
通讯作者：
Frisch, Matthias
影响因子：
3.3
作者：
Frascaroli, Elisabetta;Cane, Maria Angela;Pe, Mario Enrico
通讯作者：
Pe, Mario Enrico
影响因子：
3.3
作者：
You, AQ;Lu, XG;He, GC
通讯作者：
He, GC
影响因子：
2.3
作者：
WALEJKO, RN;RUSSELL, WA
通讯作者：
RUSSELL, WA
影响因子：
2.1
作者：
ROJAS, BA;SPRAGUE, GF
通讯作者：
SPRAGUE, GF