Recall of preexisting cross-reactive B cell memory after Omicron BA.1 breakthrough infection.
Recall of preexisting cross-reactive B cell memory after Omicron BA.1 breakthrough infection.
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Omicron BA.1 突破性感染后，回想起先前存在的交叉反应 B 细胞记忆。
DOI：
10.1126/sciimmunol.abq3511
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发表时间：
2022-07-29
影响因子：
24.8
通讯作者：
中科院分区：
文献类型：
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作者：
了解严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）突破性感染后的免疫反应将有助于开发下一代疫苗。在这里，我们分析了Omicron/BA后的spike (S)特异性B细胞反应。1感染的mrna接种供体。急性抗体反应的特点是高水平的体细胞超突变（SHM）和对祖先SARS-CoV-2菌株的识别，提示疫苗诱导的记忆B细胞（MBCs）的早期激活。BA.1突破感染诱导B细胞免疫优势结构从S2亚基（在SARS-CoV-2关注变体（VOCs）中高度保守）向抗原可变受体结合域（RBD）转移。从BA.1突破性感染供体中分离的rbd定向中和抗体大部分表现出序列趋同特征和广泛识别的SARS-CoV-2 VOCs。总之，这些发现为预先存在的免疫在形成B细胞对异源SARS-CoV-2变体暴露的反应中的作用提供了见解。ba - 1突破感染激活预先存在的记忆B细胞，对SARS-CoV-2变体具有广泛的活性。
Understanding immune responses following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) breakthrough infection will facilitate the development of next-generation vaccines. Here, we profiled spike (S)-specific B cell responses following Omicron/BA.1 infection in mRNA-vaccinated donors. The acute antibody response was characterized by high levels of somatic hypermutation (SHM) and a bias toward recognition of ancestral SARS-CoV-2 strains, suggesting the early activation of vaccine-induced memory B cells (MBCs). BA.1 breakthrough infection induced a shift in B cell immunodominance hierarchy from the S2 subunit, which is highly conserved across SARS-CoV-2 variants of concern (VOCs), and toward the antigenically variable receptor binding domain (RBD). A large proportion of RBD-directed neutralizing antibodies isolated from BA.1 breakthrough infection donors displayed convergent sequence features and broadly recognized SARS-CoV-2 VOCs. Together, these findings provide insights into the role of pre-existing immunity in shaping the B cell response to heterologous SARS-CoV-2 variant exposure. BA.1 breakthrough infection activates pre-existing memory B cells with broad activity against SARS-CoV-2 variants.
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影响因子：
14.8
作者：
Gietz, R. Daniel;Schiestl, Robert H.
通讯作者：
Schiestl, Robert H.
影响因子：
64.8
作者：
Barnes CO;Jette CA;Abernathy ME;Dam KA;Esswein SR;Gristick HB;Malyutin AG;Sharaf NG;Huey-Tubman KE;Lee YE;Robbiani DF;Nussenzweig MC;West AP Jr;Bjorkman PJ
通讯作者：
Bjorkman PJ
DOI：
10.1056/nejmoa2119451
发表时间：
2022-04-21
期刊：
The New England journal of medicine
影响因子：
--
作者：
Andrews N;Stowe J;Kirsebom F;Toffa S;Rickeard T;Gallagher E;Gower C;Kall M;Groves N;O'Connell AM;Simons D;Blomquist PB;Zaidi A;Nash S;Iwani Binti Abdul Aziz N;Thelwall S;Dabrera G;Myers R;Amirthalingam G;Gharbia S;Barrett JC;Elson R;Ladhani SN;Ferguson N;Zambon M;Campbell CNJ;Brown K;Hopkins S;Chand M;Ramsay M;Lopez Bernal J
通讯作者：
Lopez Bernal J
影响因子：
30.3
作者：
McNamara, Hayley A.;Idris, Azza H.;Cockburn, Ian A.
通讯作者：
Cockburn, Ian A.
影响因子：
24.8
作者：
Matsuda, Kenta;Huang, Jinghe;Connors, Mark
通讯作者：
Connors, Mark