Heat shock proteins in retinal neurogenesis: identification of the PM1 antigen as the chick Hsc70 and its expression in comparison to that of other chaperones
Heat shock proteins in retinal neurogenesis: identification of the PM1 antigen as the chick Hsc70 and its expression in comparison to that of other chaperones
复制标题
视网膜神经发生中的热休克蛋白：将 PM1 抗原鉴定为小鸡 Hsc70 及其与其他伴侣的表达比较
DOI：
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发表时间：
1998
影响因子：
3.4
通讯作者：
E. J. de la Rosa
中科院分区：
文献类型：
--
作者：
A. V. Morales;M. Hadjiargyrou;B. Díaz;C. Hernández;F. de Pablo;E. J. de la Rosa
虽然热休克蛋白在实验应激条件下的作用已被明确表征，但它们在非应激细胞和组织中的表达及其在正常细胞生理学中的功能，除了它们的伴侣作用外，在很大程度上仍未确定。我们在此报告了在鸡中鉴定由单克隆抗体PM 1识别的抗原[Hernández-Sánchez et al.（1994）Eur. J. Neurosci.6，1801 -1810）作为不可诱导的伴侣蛋白热休克同源物70（Hsc 70）。通过部分肽测序、免疫交叉反应性和二维凝胶电泳确定其身份。此外，我们研究了它在鸡胚视网膜神经发生过程中的表达。早期广泛的Hsc 70免疫染色对应于大多数，如果不是全部，神经上皮细胞成为限制在这些细胞的亚群在周边视网膜的发展进行。另一方面，视网膜神经节细胞，在相反的中央到外周梯度分化，保留Hsc 70免疫染色。其他分子伴侣，热休克蛋白Hsp 40，Hsp 60和Hsp 90，似乎没有补偿Hsc 70的损失。他们还表现出减少免疫染色模式的神经发生的收益，虽然不同于Hsc 70，而Hsp 70在胚胎视网膜中未检测到。Hsc 70是一种普遍认为的组成型分子伴侣，在未应激的胚胎中，这种精确的细胞和发育调控，以及其他分子伴侣的表达，提供了新的工具和对神经前体异质性的进一步了解，表明在脊椎动物神经发生过程中分子伴侣功能可能的特定细胞作用。
While the role of heat shock proteins under experimental stress conditions is clearly characterized, their expression in unstressed cells and tissues and their functions in normal cell physiology, besides their chaperone action, remain largely undetermined. We report here the identification in chicken of the antigen recognized by the monoclonal antibody PM1 [Hernández‐Sánchez et al. (1994) Eur. J. Neurosci.6,1801–1810) as the non‐inducible chaperone heat‐shock cognate 70 (Hsc70). Its identity was determined by partial peptide sequencing, immuno‐crossreactivity & two‐dimensional gel‐electrophoresis. In addition, we examined its expression during chick embryo retinal neurogenesis. The early widespread Hsc70 immunostaining corresponding to most, if not all, of the neuroepithelial cells becomes restricted to a subpopulation of these cells in the peripheral retina as development proceeds. On the other hand, retinal ganglion cells, differentiating in the opposite central‐to‐peripheral gradient, retained Hsc70 immunostaining. Other molecular chaperones, the heat‐shock proteins Hsp40, Hsp60 & Hsp90, did not seem to compensate the loss of Hsc70. They also showed decreasing immunostaining patterns as neurogenesis proceeds, although distinctive from that of Hsc70, whereas Hsp70 was not detected in the embryonic retina. This precise cellular & developmental regulation of Hsc70, a generally considered constitutive molecular chaperone, in unstressed embryos, together with the expression of other chaperones, provides new tools & a further insight on neural precursor heterogeneity & suggests possible specific cellular roles of chaperone function during vertebrate neurogenesis.
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影响因子：
2.7
作者：
MANEJWALA, FM;LOGAN, CY;SCHULTZ, RM
通讯作者：
SCHULTZ, RM
DOI：
10.1073/pnas.79.10.3218
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LI, GC;WERB, Z
通讯作者：
WERB, Z
DOI：
10.1016/0305-0491(95)02081-0
发表时间：
1996-04
期刊：
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
影响因子：
--
作者：
Adnan Ali;Luisa Salter-Cid;M. Flajnik;John J. Heikkila
通讯作者：
Adnan Ali;Luisa Salter-Cid;M. Flajnik;John J. Heikkila
影响因子：
16.2
作者：
DORSKY, RI;RAPAPORT, DH;HARRIS, WA
通讯作者：
HARRIS, WA