A novel FC17/CESA4 mutation causes increased biomass saccharification and lodging resistance by remodeling cell wall in rice.
A novel FC17/CESA4 mutation causes increased biomass saccharification and lodging resistance by remodeling cell wall in rice.
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一种新的 FC17/CESA4 突变通过重塑水稻细胞壁导致生物量糖化和抗倒伏性增加
DOI：
10.1186/s13068-018-1298-2
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发表时间：
2018
影响因子：
6.3
通讯作者：
Xu Q
中科院分区：
文献类型：
--
作者：
Li F;Liu S;Xu H;Xu Q
水稻不仅为人类生产粮食，而且还提供大量的木质纤维素残留物，最近被强调为生物燃料生产的原料。植物细胞壁的遗传修饰可以潜在地增强生物质糖化;然而，在水稻中保持正常生长并增强抗倒伏性仍然是一个挑战。在这项研究中，水稻（Oryza sativa）突变体fc 17在纤维素合成酶4（CESA 4）蛋白的植物保守区（P-CR）处具有取代（F426 S），与野生型相比，植物生长受到轻微影响，抗倒性提高了17%。更重要的是，突变体显示出生物质糖化效率提高了1.68倍。细胞壁成分分析表明，次生壁厚度和纤维素含量减少，半纤维素和木质素含量补偿性增加。X射线衍射和荧光钙染色表明，纤维素结晶度显着降低，这应该是其高糖化的关键因素。野生型和fc 17植物的蛋白质组分析进一步表明突变诱导纤维素沉积和细胞壁重塑的可能机制。这些结果表明CESA 4 P-CR位点突变影响细胞壁特征，特别是纤维素结构，从而导致生物质消化和抗倒伏性的增强。因此，CESA 4 P-CR区是细胞壁修饰的有希望的目标，以促进生物能源水稻的育种。本文的在线版本（10.1186/s13068-018-1298-2）包含补充材料，可供授权用户使用。
Rice not only produces grains for human beings, but also provides large amounts of lignocellulose residues, which recently highlighted as feedstock for biofuel production. Genetic modification of plant cell walls can potentially enhance biomass saccharification; however, it remains a challenge to maintain a normal growth with enhanced lodging resistance in rice. In this study, rice (Oryza sativa) mutant fc17, which harbors the substitution (F426S) at the plant-conserved region (P-CR) of cellulose synthase 4 (CESA4) protein, exhibited slightly affected plant growth and 17% higher lodging resistance compared to the wild-type. More importantly, the mutant showed a 1.68-fold enhancement in biomass saccharification efficiency. Cell wall composition analysis showed a reduction in secondary wall thickness and cellulose content, and compensatory increase in hemicelluloses and lignin content. Both X-ray diffraction and calcofluor staining demonstrated a significant reduction in cellulose crystallinity, which should be a key factor for its high saccharification. Proteomic profiling of wild-type and fc17 plants further indicated a possible mechanism by which mutation induces cellulose deposition and cell wall remodeling. These results suggest that CESA4 P-CR site mutation affects cell wall features especially cellulose structure and thereby causes enhancement in biomass digestion and lodging resistance. Therefore, CESA4 P-CR region is promising target for cell wall modification to facilitate the breeding of bioenergy rice. The online version of this article (10.1186/s13068-018-1298-2) contains supplementary material, which is available to authorized users.
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影响因子：
13.8
作者：
Li F;Xie G;Huang J;Zhang R;Li Y;Zhang M;Wang Y;Li A;Li X;Xia T;Qu C;Hu F;Ragauskas AJ;Peng L
通讯作者：
Peng L
DOI：
10.1073/pnas.0706569104
发表时间：
2007-09-25
影响因子：
11.1
作者：
Desprez, Thierry;Juraniec, Michal;Vernhettes, Samantha
通讯作者：
Vernhettes, Samantha
影响因子：
5.4
作者：
Hall, Melanie;Bansal, Prabuddha;Bommarius, Andreas S.
通讯作者：
Bommarius, Andreas S.
影响因子：
11.4
作者：
Li, Ming;Si, Shengli;Peng, Liangcai
通讯作者：
Peng, Liangcai
影响因子：
7.7
作者：
Ralph, John;Lundquist, Knut;Boerjan, Wout
通讯作者：
Boerjan, Wout