Distribution of immune cells in the human cervix and implications for HIV transmission.
Distribution of immune cells in the human cervix and implications for HIV transmission.
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DOI：
10.1111/aji.12198
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发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
van Baarle D
中科院分区：
文献类型：
--
作者：
Trifonova RT;Lieberman J;van Baarle D
了解人类女性生殖道粘膜免疫细胞组成对了解HIV-1易感性非常重要。我们开发了一种优化的程序，用于对人宫颈免疫细胞进行多色流式细胞术分析，以表征宫颈内和宫颈外的所有主要免疫细胞亚群。一半的组织造血细胞为CD14+，其中巨噬细胞居多，约三分之一的组织造血细胞为CD11c+，其中大部分为CD103-CD11b+CX3CR1+DC-SIGN+树突状细胞（dc）。另一个优势群体是T细胞，CD8多于CD4细胞。绝经前妇女子宫颈的T细胞（CD8和CD4）和B细胞比宫颈内丰富，但绝经后子宫颈的CD8+ T细胞和B细胞数量下降，而CD4 T细胞计数仍然较高。B、NK和常规髓系和浆系dc各占组织造血细胞的百分之几。尽管宫颈外有更多的hiv易感CD4+ T细胞，极化宫颈内外植体明显更好地支持hiv复制。由于它们在生殖道中的丰富，CX3CR1+DC-SIGN+ dc可能在hiv传播中很重要。我们的数据还表明，上生殖道的柱状上皮可能是hiv传播的首选部位。
Knowledge of the mucosal immune cell composition of the human female genital tract is important for understanding susceptibility to HIV-1. We developed an optimized procedure for multicolor flow cytometry analysis of immune cells from human cervix to characterize all major immune cell subsets in the endocervix and ectocervix. Half of tissue hematopoietic cells were CD14+, many of which were macrophages and about a third were CD11c+, most of which were CD103-CD11b+CX3CR1+DC-SIGN+ dendritic cells (DCs). The other dominant population were T cells, with more CD8 than CD4 cells. T cells (both CD8 and CD4) and B cells were more abundant in the ectocervix than endocervix of premenopausal women, however CD8+ T cell and B cell numbers declined in the ectocervix after menopause, while CD4 T cell counts remained higher. B, NK and conventional myeloid and plasmocytoid DCs each were a few percent of tissue hematopoietic cells. Although the ectocervix had more HIV-susceptible CD4+ T cells, polarized endocervical explants supported HIV-replication significantly better. Due to their abundance in the genital tract CX3CR1+DC-SIGN+DCs might be important in HIV-transmission. Our data also suggests that the columnar epithelium of the upper genital tract might be a preferential site for HIV-transmission.
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影响因子：
64.8
作者：
Cella, Marina;Fuchs, Anja;Vermi, William;Facchetti, Fabio;Otero, Karel;Lennerz, Jochen K. M.;Doherty, Jason M.;Mills, Jason C.;Colonna, Marco
通讯作者：
Colonna, Marco
影响因子：
3.4
作者：
Hickey, D. K.;Patel, M. V.;Fahey, J. V.;Wira, C. R.
通讯作者：
Wira, C. R.
DOI：
10.1084/jem.20041196
发表时间：
2004-09-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Mehandru S;Poles MA;Tenner-Racz K;Horowitz A;Hurley A;Hogan C;Boden D;Racz P;Markowitz M
通讯作者：
Markowitz M
影响因子：
32.4
作者：
Klechevsky, Eynav;Morita, Rimpei;Liu, Maochang;Cao, Yanying;Coquery, Sebastien;Thompson-Snipes, LuAnn;Briere, Francine;Chaussabel, Damien;Zurawski, Gerard;Palucka, A. Karolina;Reiter, Yoram;Banchereau, Jacques;Ueno, Hideki
通讯作者：
Ueno, Hideki
影响因子：
15.8
作者：
Denton PW;Estes JD;Sun Z;Othieno FA;Wei BL;Wege AK;Powell DA;Payne D;Haase AT;Garcia JV
通讯作者：
Garcia JV