Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum
Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum
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病原菌鳗弧菌fosmid文库的构建及表征
DOI：
10.1007/s00343-009-9230-z
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发表时间：
2009-11
期刊：
影响因子：
--
通讯作者：
Mo Zhaolan
中科院分区：
文献类型：
--
作者：
Li Jie;Mao Yunxiang;Hao Bin;Guo Dongsheng;Han Yifan;Xiao Peng;Mo Zhaolan
鳗弧菌是一种常见的鱼类致病菌。然而，人们对其致病机制知之甚少，部分原因是整个基因组还没有完全测序。我们构建了包含960个克隆的鳗弧菌Fosmid文库，平均插入大小为37.7kb，基因组覆盖率为8.6倍。我们通过对随机选择的50个克隆进行末端测序来鉴定文库。这产生了93个序列，总长度为57485bp，覆盖了整个基因组的1.4%。在这些序列中，58个(62.4%)与已知基因同源，30个(32.3%)具有假设功能的基因，其余5个(5.3%)为未知基因。通过对10个基因的PCR筛选，验证了该文库的实用性。这导致平均每一次筛查有6.2个Fosmid克隆。
Vibrio anguillarum is a common bacterial pathogen in fish. However, little is known about its pathogenic mechanism, in part, because the entire genome has not been completely sequenced. We constructed a fosmid library for V. anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage. We characterized the library by end-sequencing 50 randomly selected clones. This generated 93 sequences with a total length of 57 485 bp covering 1.4% of the whole genome. Of these sequences, 58 (62.4%) were homologous to known genes, 30 (32.3%) were genes with hypothetical functions, and the remaining 5 (5.3%) were unknown genes. We demonstrated the utility of this library by PCR screening of 10 genes. This resulted in an average of 6.2 fosmid clones per screening.
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影响因子：
3.5
作者：
M. Macdonell;R. Colwell
通讯作者：
M. Macdonell;R. Colwell
影响因子：
--
作者：
Z. Mo;Shiqiong Chen;Pei-jun Zhang
通讯作者：
Z. Mo;Shiqiong Chen;Pei-jun Zhang
基于fosmid文库的极大节旋藻序列分析
影响因子：
3.3
作者：
Zhang, Xuecheng;Wang, Guangce;Mo, Zhaolan;Liu, Wei;Mao, Yunxiang;Ling, Na
通讯作者：
Ling, Na
影响因子：
3.1
作者：
Jenelle D. F. Meyer;Wim Deleu;J. Garcia-Mas;M. Havey
通讯作者：
Jenelle D. F. Meyer;Wim Deleu;J. Garcia-Mas;M. Havey
影响因子：
5.2
作者：
Liang, CY;Xi, Y;Wang, B
通讯作者：
Wang, B