Eukaryotic association module in phage WO genomes from Wolbachia.
Eukaryotic association module in phage WO genomes from Wolbachia.
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DOI：
10.1038/ncomms13155
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发表时间：
2016-10-11
影响因子：
16.6
通讯作者：
Bordenstein, Seth R.
中科院分区：
文献类型：
--
作者：
Bordenstein, Sarah R.;Bordenstein, Seth R.
病毒分为真核生物、古细菌和细菌三类。这种特定领域的生态学强调了为什么真核病毒通常会吸收真核基因，而噬菌体通常会携带细菌基因。然而，噬菌体在真核生物的专性胞内细菌中的存在可能促进真核生物和噬菌体之间的DNA转移。在这里，我们报告了纯化的噬菌体WO颗粒的沃尔巴克氏体的宏基因组分析，并揭示了在完整的WO基因组真核关联模块。它含有预测的结构域，如黑寡妇latrotoxin C-末端结构域，其在噬菌体基因组中是不间断的，富含真核蛋白酶切割位点，并与其他结构域组合以形成迄今为止最大的噬菌体基因之一（14，256 bp）。据我们所知，这些真核生物样结构域以前从未在包装的噬菌体中报道过，它们的同源性、分布和序列多样性意味着噬菌体/原噬菌体和动物基因组之间的横向转移。最后，WO基因组序列和附着位点的鉴定将潜在地推进沃尔巴克氏体的遗传操作。 病毒通常与宿主交换遗传物质，但不与其他生命领域的物种交换。在这里，作者发现沃尔巴克氏体的噬菌体WO含有真核生物样基因，暗示真核生物和感染细菌的病毒之间的横向遗传转移。
Viruses are trifurcated into eukaryotic, archaeal and bacterial categories. This domain-specific ecology underscores why eukaryotic viruses typically co-opt eukaryotic genes and bacteriophages commonly harbour bacterial genes. However, the presence of bacteriophages in obligate intracellular bacteria of eukaryotes may promote DNA transfers between eukaryotes and bacteriophages. Here we report a metagenomic analysis of purified bacteriophage WO particles of Wolbachia and uncover a eukaryotic association module in the complete WO genome. It harbours predicted domains, such as the black widow latrotoxin C-terminal domain, that are uninterrupted in bacteriophage genomes, enriched with eukaryotic protease cleavage sites and combined with additional domains to forge one of the largest bacteriophage genes to date (14,256 bp). To the best of our knowledge, these eukaryotic-like domains have never before been reported in packaged bacteriophages and their phylogeny, distribution and sequence diversity imply lateral transfers between bacteriophage/prophage and animal genomes. Finally, the WO genome sequences and identification of attachment sites will potentially advance genetic manipulation of Wolbachia. Viruses commonly exchange genetic material with their hosts, but not with species from other domains of life. Here, the authors find that the bacteriophage WO of Wolbachia contains eukaryotic-like genes, implicating lateral genetic transfer between eukaryotes and viruses infecting bacteria.
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发表时间：
2004-05-14
影响因子：
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