Postnatal development of a brain-specific subspecies of protein kinase C in rat-猫眼课题宝

大鼠脑特异性蛋白激酶 C 亚种的出生后发育

DOI：

10.1523/jneurosci.08-05-01678.1988

发表时间：

1988

期刊：

British Journal of Pharmacology

影响因子：

7.3

通讯作者：

中科院分区：

医学2区

文献类型：

作者：

Takeshi Hashimoto;Katsuhiko Ase;S. Sawamura;U. Kikkawa;Naoaki Saito;Chikako Tanaka;Yasutomi Nishizuka

蛋白激酶C在发育中的大鼠脑进行了研究，通过生化测定和光镜免疫细胞化学。蛋白激酶在羟基磷灰石柱层析上被分解成3个级分，指定为I、II和III型。I型结构由γ序列编码，在产后早期未被检测到，在第一周保持低水平的活动，然后逐渐增加，并在产后第28天左右达到最大值。这种类型的酶在神经组织中特异性表达，并且在迄今为止测试的任何其他组织中未发现。II型酶活性，由β I-和β II-序列编码的2个亚种的混合物，在出生时被发现，迅速增加，并在出生后第14天和第28天之间达到平台水平。这种类型是脑中蛋白激酶C的主要亚种。III型，其结构编码的α序列，也检测到出生时，并达到其最高水平的出生后第7天。用单克隆抗体进行的免疫细胞化学研究显示了浦肯野细胞中I型亚种的发育模式，该抗体优先识别I型酶，浦肯野细胞是具有大量I型蛋白激酶C的典型细胞。

Protein kinase C in the developing rat brain was investigated by a biochemical assay and by light-microscopic immunocytochemistry. The protein kinase was resolved on hydroxyapatite column chromatography into 3 fractions, designated types I, II, and III. Type I, with structure encoded by a gamma-sequence, was not detected early postnatally, maintained a low level of activity during the first week, which increased gradually, and reached its maximum around postnatal day 28. This type of enzyme was expressed specifically in nervous tissues, and was not found in any other tissues thus far tested. Type II enzyme activity, a mixture of the 2 subspecies encoded by the beta I- and beta II-sequences, was found at birth, increased rapidly, and reached a plateau level between postnatal days 14 and 28. This type was the predominant subspecies of protein kinase C in the brain. Type III, its structure encoded by the alpha-sequence, was also detected at birth, and reached its maximum level on postnatal day 7. Immunocytochemical studies with a monoclonal antibody, which recognized preferentially the type I enzyme, visualized the developmental pattern of type I subspecies in the Purkinje cell, a typical cell having a large quantity of type I protein kinase C.