Anti-inflammatory effect of hydroxyproline-GQDGLAGPK in desiccation stress-induced experimental dry eye mouse.
Anti-inflammatory effect of hydroxyproline-GQDGLAGPK in desiccation stress-induced experimental dry eye mouse.
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DOI：
10.1038/s41598-017-07965-4
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发表时间：
2017-08-07
影响因子：
4.6
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Lee H;Kim CE;Ahn BN;Yang J
本研究的目的是研究新型肽羟脯氨酸-GQDGLAGPK（Hyp-GQDGLAGPK）在干燥应激诱导的干眼小鼠模型中的作用，并比较环孢素、地夸磷索和透明质酸钠等治疗干眼病的药物。 78只NOD.B10.H2 b小鼠注射东莨菪碱并暴露在空气中10 d，然后用生理盐水（n = 13）、1% Hyp-GQDGLAGPK（n = 13）、0.05%环孢素（n = 13）、3%地夸磷索处理。 (n = 13) 和 0.1% 透明质酸 (n = 13) 10 天。在 DS 10 天时，使用 13 只小鼠进行组织病理学分析。干燥应激显着降低了泪液产生，但 Hyp-GQDGLAGPK 的局部治疗恢复至基线水平，与环孢素和地夸磷索相似。此外，Hyp-GQDGLAGPK 改善了促进上皮稳定的作用，包括角膜不规则评分、荧光素评分和角膜上皮脱离。这些角膜上皮稳定性的改善优于环孢菌素和透明质酸钠组。此外，干燥应激显着诱导泪腺中自身免疫炎症相关因子的表达，但它被 Hyp-GQDGLAGPK 治疗显着抑制。总的来说，我们发现新型肽Hyp-GQDGLAGPK具有稳定泪膜和抑制炎症等多功能作用。
The purpose of this study has been the investigation of the effect of novel peptide hydroxyproline-GQDGLAGPK (Hyp-GQDGLAGPK) in desiccation stress-induced dry eye mouse model and compared medicines for dry eye disease including cyclosporine, diquafosol and sodium hyaluronate. Seventy eight NOD.B10.H2 b mice were injected with scopolamine and exposed to an air draft for 10 days, and then the mice were treated with normal saline (n = 13), 1% Hyp-GQDGLAGPK (n = 13), 0.05% cyclosporine (n = 13), 3% diquafosol (n = 13), and 0.1% hyaluronate (n = 13) for 10 days. Thirteen mice were used for histopathologic analysis at DS 10d. The desiccation stress significantly decreased tear production, but the topical treatment of Hyp-GQDGLAGPK recovered to the baseline levels, which was similar to cyclosporine and diquafosol. In addition, Hyp-GQDGLAGPK improved facilitating epithelium stabilization including the corneal irregularity score, fluorescein score and detachment of the corneal epithelium. These improvements in stabilization of the corneal epithelium was superior to that in the cyclosporine and sodium hyaluronate groups. Furthermore, desiccation stress markedly induced expression of autoimmune inflammation-related factors in the lacrimal glands, but it was significantly suppressed by Hyp-GQDGLAGPK treatment. Overall, we found that novel peptide Hyp-GQDGLAGPK has multi-functional effects such as stabilizing the tear film and inhibiting inflammation.
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影响因子：
4.4
作者：
Chotikavanich S;de Paiva CS;Li de Q;Chen JJ;Bian F;Farley WJ;Pflugfelder SC
通讯作者：
Pflugfelder SC
影响因子：
3.4
作者：
Gao, JP;Morgan, G;Stern, ME
通讯作者：
Stern, ME
DOI：
10.2147/opth.s39699
发表时间：
2014
期刊：
Clinical ophthalmology (Auckland, N.Z.)
影响因子：
--
作者：
Lau OC;Samarawickrama C;Skalicky SE
通讯作者：
Skalicky SE
影响因子：
2.3
作者：
Fujihara, T;Murakami, T;Nakata, K
通讯作者：
Nakata, K
DOI：
10.2147/opth.s69486
发表时间：
2015
期刊：
Clinical ophthalmology (Auckland, N.Z.)
影响因子：
--
作者：
Koh S
通讯作者：
Koh S