Structural and functional studies of membrane remodeling machines.
Structural and functional studies of membrane remodeling machines.
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DOI:
10.1016/bs.mcb.2015.02.007
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发表时间:
2015
影响因子:
--
通讯作者:
Frost A
中科院分区:
文献类型:
--
作者:
Kalia R;Talledge N;Frost A
Building cells from their component parts will hinge upon our ability to reconstitute biochemical compartmentalization and exchange between membrane-delimited organelles. By contrast with our understanding of other cellular events, the mechanisms that govern membrane trafficking has lagged because the presence of phospholipid bilayers complicates the use of standard methods. This chapter describes in vitro methods for purifying, reconstituting and visualizing membrane remodeling activities directly by electron cryomicroscopy (cryoEM).
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