Additively manufactured 3D porous Ti-6Al-4V constructs mimic trabecular bone structure and regulate osteoblast proliferation, differentiation and local factor production in a porosity and surface roughness dependent manner.
Additively manufactured 3D porous Ti-6Al-4V constructs mimic trabecular bone structure and regulate osteoblast proliferation, differentiation and local factor production in a porosity and surface roughness dependent manner.
复制标题
DOI：
10.1088/1758-5082/6/4/045007
复制
发表时间：
2014-10-07
期刊：
影响因子：
9
通讯作者：
Schwartz Z
中科院分区：
文献类型：
--
作者：
Cheng A;Humayun A;Cohen DJ;Boyan BD;Schwartz Z
通过激光烧结的增材制造能够生产用于骨科和牙科植入物的高分辨率金属结构。在本研究中，我们使用人骨小梁模板通过激光烧结设计和制造具有不同孔隙率的Ti-6Al-4V结构。结构的表征显示了15-70%的互连孔隙率，压缩模量为2063-2954 MPa。这些具有大孔隙率的结构进一步进行表面处理，以产生所需的多尺度微米/纳米粗糙度，这已被证明可以增强骨整合过程。成骨细胞（MG 63细胞）在构建体上生长时表现出高活力。增殖（DNA）和碱性磷酸酶比活性（ALP），早期分化标志物，随着孔隙度的增加而下降，而骨钙素（OCN），晚期分化标志物，以及骨保护素（OPG），血管内皮生长因子（VEGF）和骨形态发生蛋白2和4（BMP 2，BMP 4）随着孔隙度的增加而增加。具有最高孔隙率和表面改性的3D结构支持最大的成骨细胞分化和局部因子产生。这些结果表明，模拟人骨小梁并经额外表面处理生产的增材制造3D多孔结构可定制用于增加成骨细胞反应。在高孔隙率结构上成骨细胞成熟和分化的增加的因素表明这些表面在体内增加骨整合的增强的性能。
Additive manufacturing by laser sintering is able to produce high resolution metal constructs for orthopaedic and dental implants. In this study, we used a human trabecular bone template to design and manufacture Ti-6Al-4V constructs with varying porosity via laser sintering. Characterization of constructs revealed interconnected porosities ranging from 15–70% with compressive moduli of 2063–2954 MPa. These constructs with macro porosity were further surface-treated to create a desirable multi-scale micro-/nano-roughness, which has been shown to enhance the osseointegration process. Osteoblasts (MG63 cells) exhibited high viability when grown on the constructs. Proliferation (DNA) and alkaline phosphatase specific activity (ALP), an early differentiation marker, decreased as porosity increased, while osteocalcin (OCN), a late differentiation marker, as well as osteoprotegerin (OPG), vascular endothelial growth factor (VEGF) and bone morphogenetic proteins 2 and 4 (BMP2, BMP4) increased with increasing porosity. 3D constructs with the highest porosity and surface modification supported the greatest osteoblast differentiation and local factor production. These results indicate that additively manufactured 3D porous constructs mimicking human trabecular bone and produced with additional surface treatment can be customized for increased osteoblast response. Increased factors for osteoblast maturation and differentiation on high porosity constructs suggest the enhanced performance of these surfaces for increasing osseointegration in vivo.
登录
查看更多内容
DOI：
10.1073/pnas.0903269107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Kilian, Kristopher A.;Bugarija, Branimir;Mrksich, Milan
通讯作者：
Mrksich, Milan
影响因子：
14
作者：
Hollander, DA;von Walter, M;Erli, HJ
通讯作者：
Erli, HJ
DOI：
10.1302/0301-620x.84b6.11994
发表时间：
2002-08-01
影响因子：
--
作者：
Ding, M;Odgaard, A;Hvid, I
通讯作者：
Hvid, I
DOI：
10.1073/pnas.0805420105
发表时间：
2008-10-14
影响因子：
11.1
作者：
Olivares-Navarrete, R.;Raz, P.;Schwartz, Z.
通讯作者：
Schwartz, Z.
影响因子：
8.4
作者：
Bertol, Liciane Sabadin;Kindlein Junior, Wilson;Aumund-Kopp, Claus
通讯作者：
Aumund-Kopp, Claus