Comparative studies to determine the efficiency of 6 methylpurine deoxyriboside to detect cell culture mycoplasmas.
Comparative studies to determine the efficiency of 6 methylpurine deoxyriboside to detect cell culture mycoplasmas.
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比较研究确定 6 甲基嘌呤脱氧核苷检测细胞培养支原体的效率。
DOI：
10.1007/bf02623401
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Carson,D
中科院分区：
文献类型：
--
作者：
McGarrity,GJ;Kotani,H;Carson,D
Studies were performed to compare three methods to detect mycoplasmal infection of cell cultures. The methods included microbiological assay by inoculation into broth and onto agar with anaerobic incubation, fluorescent DNA staining by Hoechst 33258, and mycoplasmal mediated cytotoxicity by 6 methylpurine deoxyriboside (6MPDR). Fluorescent DNA staining and 6MPDR assays were performed in an indicator cell culture system. A total of 2589 cell cultures were assayed. Mycoplasmas were detected in 174, an incidence of 6.7%. Species isolated were:Acholeplasma laidlawii, Mycoplasma orale, M. arginini, M. hyorhinis, M. fermentans, M. pirum, and M. pneumoniae. In separate studies, 6MPDR also detected infection withSpiroplasma mirumwhen this organisms was deliberately inoculated into cell cultures. The efficiencies of microbiological testing, fluorescent DNA assays, and 6MPDR were 43.1, 98. 8, and 97.1%, respectively.
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血管阻力的中枢神经调节。
影响因子：
18.2
作者：
S. Hilton;K. Spyer
通讯作者：
K. Spyer
对孤束核的化学刺激以及由此产生的血压反应。
影响因子：
3
作者：
W. de Jong;M. Petty
通讯作者：
M. Petty
苯乙醇胺 N-甲基转移酶和多巴胺-β-羟化酶抑制对大脑中肾上腺素水平的影响。
影响因子：
6.1
作者：
A. Sauter;J. Y. Lew;Y. Baba;M. Goldstein
通讯作者：
M. Goldstein
DOI：
--
发表时间：
1941
期刊：
影响因子：
--
作者：
R. Pitts;M. Larrabee;D. W. Bronk
通讯作者：
D. W. Bronk
肾血管性高血压发生过程中脑干和下丘脑核团去甲肾上腺素浓度的变化
影响因子：
2.9
作者：
M. Petty;J. Reid
通讯作者：
J. Reid