Evaluation of Nanopore Sensor Design Using Electrical and Optical Analyses.
Evaluation of Nanopore Sensor Design Using Electrical and Optical Analyses.
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DOI：
10.1021/acsnano.3c02532
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发表时间：
2023-06-13
期刊：
影响因子：
17.1
通讯作者：
Movileanu, Liviu
中科院分区：
文献类型：
--
作者：
Mayse, Lauren A.;Imran, Ali;Wang, Yazheng;Ahmad, Mohammad;Oot, Rebecca A.;Wilkens, Stephan;Movileanu, Liviu
关键词：
纳米孔目前被用作单分子蛋白质传感的有力工具。报告信号通常需要蛋白质分析物进入纳米孔内部，但已经出现了一类这样的传感器，其允许在溶液中自由进行靶向检测。这种策略消除了纳米孔限制的空间限制。然而，探测纳米孔外的蛋白质意味着与将水相中的物理相互作用转换为可靠的电特征相关的许多挑战。因此，它需要大量的工程和繁琐的优化路线。这些障碍阻碍了这些传感器的广泛采用。在这里，我们提供了一个实验策略，通过开发和验证单多肽链纳米孔服从单分子和散装相蛋白质检测方法。我们利用蛋白质工程，以及纳米孔和纳米盘技术，创造纳米孔传感器，除了传统的电子记录，还可以与光学平台集成。在探测器集合上使用光学模态加速了这些传感器针对特定任务的优化过程。它还提供了这些单分子纳米孔传感器的构建如何影响其性能的见解。这些结果形成了评估工程纳米孔超出连续脉冲技术的基本限制的基础。
Nanopores are currently utilized as powerful tools for single-molecule protein sensing. The reporting signal typically requires protein analytes to enter the nanopore interior, yet a class of these sensors has emerged that allows targeted detection free in solution. This tactic eliminates the spatial limitation of nanopore confinement. However, probing proteins outside the nanopore implies numerous challenges associated with transducing the physical interactions in the aqueous phase into a reliable electrical signature. Hence, it necessitates extensive engineering and tedious optimization routes. These obstacles have prevented the widespread adoption of these sensors. Here, we provide an experimental strategy by developing and validating single-polypeptide-chain nanopores amenable to single-molecule and bulk-phase protein detection approaches. We utilize protein engineering, as well as nanopore and nanodisc technologies, to create nanopore sensors that can be integrated with an optical platform in addition to traditional electrical recordings. Using the optical modality over an ensemble of detectors accelerates these sensors’ optimization process for a specific task. It also provides insights into how the construction of these single-molecule nanopore sensors influences their performance. These outcomes form a basis for evaluating engineered nanopores beyond the fundamental limits of the resistive-pulse technique.
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影响因子：
16.1
作者：
Chen, Xiaoying;Zaro, Jennica L.;Shen, Wei-Chiang
通讯作者：
Shen, Wei-Chiang
影响因子：
17.1
作者：
Fahie M;Chisholm C;Chen M
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Chen M
影响因子：
16.6
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Galenkamp NS;Soskine M;Hermans J;Wloka C;Maglia G
通讯作者：
Maglia G
影响因子：
15
作者：
Ensslen, Tobias;Sarthak, Kumar;Behrends, Jan C.
通讯作者：
Behrends, Jan C.
影响因子：
16.6
作者：
Harrington, Leon;Alexander, Leila T.;Bayley, Hagan
通讯作者：
Bayley, Hagan