Cross-kingdom RNA trafficking and environmental RNAi for powerful innovative pre- and post-harvest plant protection.
Cross-kingdom RNA trafficking and environmental RNAi for powerful innovative pre- and post-harvest plant protection.
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DOI：
10.1016/j.pbi.2017.05.003
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发表时间：
2017-08
影响因子：
9.5
通讯作者：
Jin H
中科院分区：
文献类型：
--
作者：
Wang M;Thomas N;Jin H
小RNA（sRNA）诱导的RNA干扰（RNAi）是几乎所有真核生物中调节基因表达的重要保守机制。一些sRNA在细胞间短距离移动，而一些sRNA则长距离传播到整个生物体。最近的研究表明，sRNA甚至可以在生物体之间移动以诱导基因沉默，这种现象称为“跨王国RNAi”。病原体、害虫或共生体与其各自宿主之间的sRNA运输可对相互作用相容性具有显著影响。发现某些sRNA从病原体或害虫进入宿主细胞并抑制宿主免疫以在植物和动物中实现成功感染;而从宿主细胞产生的sRNA也易位到病原体或寄生虫细胞中以抑制其毒力。这种跨界RNAi机制使得能够使用靶向病原体和害虫的必需基因的植物来源的RNA开发有效的疾病控制方法。此外，最近在某些真菌病原体中发现了从环境中摄取外源RNA，这使得可以通过将病原体靶向RNA直接应用于作物和收获后产品来抑制真菌疾病，以避免广泛的化学处理并避免产生转基因植物。这种喷雾诱导的基因沉默（SIGS）策略是环境可持续的和友好的，并且可以很容易地适应于同时控制多种真菌疾病。
Small RNA (sRNA)-induced RNA interference (RNAi) is an important conserved mechanism that modulates gene expression in almost all eukaryotes. Some sRNAs move short distances from cell to cell, while some travel long distances to spread systemically throughout the organism. Recent studies indicate that sRNAs can even move between organisms to induce gene silencing, a phenomenon called “cross-kingdom RNAi”. sRNA trafficking between a pathogen, pest, or symbiont and its respective host can have a significant impact on interaction compatibility. Certain sRNAs were found to travel from pathogens or pests into host cells and suppress host immunity to achieve successful infection in both plants and animals; while sRNAs generated from host cells also translocate into pathogen or parasite cells to inhibit their virulence. Such cross-kingdom RNAi mechanisms enable the development of efficient disease control methods using plant-derived RNAs that target essential genes of pathogens and pests. Moreover, uptake of exogenous RNAs from the environment was recently discovered in certain fungal pathogens, which makes it possible to suppress fungal diseases by directly applying pathogen–targeting RNAs on crops and post-harvested products to avoid extensive chemical treatment and circumvent generating genetically modified plants. This spray-induced gene silencing (SIGS) strategy is environmentally sustainable and friendly, and can be easily adapted to control multiple fungal diseases simultaneously.
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DOI：
10.1261/rna.048116.114
发表时间：
2015-05
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Ivashuta S;Zhang Y;Wiggins BE;Ramaseshadri P;Segers GC;Johnson S;Meyer SE;Kerstetter RA;McNulty BC;Bolognesi R;Heck GR
通讯作者：
Heck GR
影响因子：
11.2
作者：
Katakowski M;Buller B;Wang X;Rogers T;Chopp M
通讯作者：
Chopp M
DOI：
10.1073/pnas.1201153109
发表时间：
2012-09-04
影响因子：
11.1
作者：
Jose, Antony M.;Kim, Yunsoo A.;Hunter, Craig P.
通讯作者：
Hunter, Craig P.
影响因子：
56.9
作者：
Feinberg, EH;Hunter, CP
通讯作者：
Hunter, CP
DOI：
10.1073/pnas.0604698103
发表时间：
2006-09-26
影响因子：
11.1
作者：
Huang, Guozhong;Allen, Rex;Hussey, Richard S.
通讯作者：
Hussey, Richard S.