ER network dynamics are differentially controlled by myosins XI-K, XI-C, XI-E, XI-I, XI-1, and XI-2.

ER network dynamics are differentially controlled by myosins XI-K, XI-C, XI-E, XI-I, XI-1, and XI-2.
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DOI:
10.3389/fpls.2014.00218
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发表时间:
2014
影响因子:
5.6
通讯作者:
Sparkes I
Sparkes I
中科院分区:
生物学2区
文献类型:
--
作者:
Griffing LR;Gao HT;Sparkes I

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高等植物的内质网(ER)是一个由小管和池组成的复杂网络。一些肾小管和脑池相对持久,而另一些则通过生长和收缩、肾小管伸长和收缩的循环以及脑池扩张和缩小来动态移动和重塑。先前的研究表明,肌球蛋白 XI-K 的无运动、截短尾部在烟草叶中的瞬时表达增加了内质网中持久池和小管的相对面积。同样,XI-K 尾部的瞬时表达会减弱高尔基体和过氧化物酶体等细胞器的运动。为了检查其他 XI 类肌球蛋白是否参与 ER 的重塑和运动,其他与细胞器运动有关的肌球蛋白 XI,XI-1 (MYA1)、XI-2 (MYA2)、XI-C、XI-E、XI-I 和一个不参与的 XI-A,被表达为无运动尾部结构,并确定它们对 ER 持久结构的影响。在这里,我们指出了对 ER 动力学的不同影响,即某些 XI 类肌球蛋白可能对控制池化而不是管状化具有更大的影响。
The endoplasmic reticulum (ER) of higher plants is a complex network of tubules and cisternae. Some of the tubules and cisternae are relatively persistent, while others are dynamically moving and remodeling through growth and shrinkage, cycles of tubule elongation and retraction, and cisternal expansion and diminution. Previous work showed that transient expression in tobacco leaves of the motor-less, truncated tail of myosin XI-K increases the relative area of both persistent cisternae and tubules in the ER. Likewise, transient expression of XI-K tail diminishes the movement of organelles such as Golgi and peroxisomes. To examine whether other class XI myosins are involved in the remodeling and movement of the ER, other myosin XIs implicated in organelle movement, XI-1 (MYA1),XI-2 (MYA2), XI-C, XI-E, XI-I, and one not, XI-A, were expressed as motor-less tail constructs and their effect on ER persistent structures determined. Here, we indicate a differential effect on ER dynamics whereby certain class XI myosins may have more influence over controlling cisternalization rather than tubulation.
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