Myosin XIK of Arabidopsis thaliana accumulates at the root hair tip and is required for fast root hair growth.
Myosin XIK of Arabidopsis thaliana accumulates at the root hair tip and is required for fast root hair growth.
复制标题
DOI：
10.1371/journal.pone.0076745
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nebenführ A
中科院分区：
文献类型：
--
作者：
Park E;Nebenführ A
肌球蛋白马达蛋白被认为通过沿肌动蛋白细胞骨架移动细胞器、小泡或蛋白质复合体等细胞成分，在细胞极性的建立和维持中发挥重要作用。在拟南芥中，肌球蛋白xik基因的破坏导致高极性根毛的伸长减少，这表明编码的马达蛋白参与了这种细胞的生长。在这项研究中，详细的活细胞观察显示，与野生型相比，西克根毛的伸长速度更慢，停止生长的速度更快。包括肌动蛋白细胞骨架在内的整体细胞结构看起来正常，但突变体的肌动蛋白细丝动力学降低。另一方面，含有RabA4b的囊泡的积累与野生型没有显著差异。一个功能性的YFP-xik融合蛋白可以补充突变的表型，以肌动蛋白依赖的方式积累在生长的根毛顶端。然而，YFP-xik在末端的分布与ER或包括CFP-RabA4b在内的几个末端丰富的标记不匹配。我们的结论是，肌球蛋白xik是正常肌动蛋白动力学所必需的，并在生长的根毛的根尖下区域发挥作用，以促进最佳生长。
Myosin motor proteins are thought to carry out important functions in the establishment and maintenance of cell polarity by moving cellular components such as organelles, vesicles, or protein complexes along the actin cytoskeleton. In Arabidopsis thaliana, disruption of the myosin XIK gene leads to reduced elongation of the highly polar root hairs, suggesting that the encoded motor protein is involved in this cell growth. Detailed live-cell observations in this study revealed that xik root hairs elongated more slowly and stopped growth sooner than those in wild type. Overall cellular organization including the actin cytoskeleton appeared normal, but actin filament dynamics were reduced in the mutant. Accumulation of RabA4b-containing vesicles, on the other hand, was not significantly different from wild type. A functional YFP-XIK fusion protein that could complement the mutant phenotype accumulated at the tip of growing root hairs in an actin-dependent manner. The distribution of YFP-XIK at the tip, however, did not match that of the ER or several tip-enriched markers including CFP-RabA4b. We conclude that the myosin XIK is required for normal actin dynamics and plays a role in the subapical region of growing root hairs to facilitate optimal growth.
登录
查看更多内容
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
2.7
作者：
Jiang, Shu-Ye;Cai, Minnie;Ramachandran, Srinivasan
通讯作者：
Ramachandran, Srinivasan
影响因子：
2.9
作者：
Hoffmann, A;Nebenführ, A
通讯作者：
Nebenführ, A
Lifeact 的应用揭示了拟南芥和地钱地钱中的 F-肌动蛋白动态。
影响因子：
4.9
作者：
Era, Atsuko;Tominaga, Motoki;Ebine, Kazuo;Awai, Chie;Saito, Chieko;Ishizaki, Kimitsune;Yamato, Katsuyuki T.;Kohchi, Takayuki;Nakano, Akihiko;Ueda, Takashi
通讯作者：
Ueda, Takashi
影响因子：
64.8
作者：
Carol, RJ;Takeda, S;Dolan, L
通讯作者：
Dolan, L