Genomic and functional analysis of phage-mediated horizontal gene transfer in Pseudomonas syringae on the plant surface.
Genomic and functional analysis of phage-mediated horizontal gene transfer in Pseudomonas syringae on the plant surface.
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DOI：
10.1111/nph.18573
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发表时间：
2023-02
期刊：
影响因子：
9.4
通讯作者：
Harrison, Richard J.
中科院分区：
文献类型：
--
作者：
Hulin, Michelle T.;Rabiey, Mojgan;Zeng, Ziyue;Dieguez, Andrea Vadillo;Bellamy, Sophia;Swift, Phoebe;Mansfield, John W.;Jackson, Robert W.;Harrison, Richard J.
关键词：
许多假单胞菌菌株定殖在植物表面，包括樱桃溃烂病病原菌、紫丁香假单胞菌致病变种和桑枝霉。我们研究了樱桃叶球体中丁香疫霉菌的基因组多样性，并重点研究了前驱体在编码3型分泌效应蛋白(T3SE)基因转移中的作用，这些蛋白有助于毒力的进化。对英国果园附生假单胞菌进行了系统发育分析。附生植物种群在不同地区之间存在显著差异。非致病株含有T3SE基因库。系统群4和10的成员均为首次从李属植物中鉴定出来。利用生物信息学，我们探索了编码T3SE HopAR1的基因在包括樱桃附生真菌和病原菌在内的不同的紫丁香菌菌株的相关噬菌体序列中的存在。结果表明，该效应子的水平基因转移(HGT)可能与噬菌体有关。含有HopAR1的原噬菌体被证实在叶表面切除、循环和转移该基因。叶球为原噬菌体介导的基因交换提供了一个动态环境，并为出现新的更具毒力的致病类型提供了可能性。我们的结果表明，对环境人口进行基于基因组的流行病学监测将使及时应用控制措施来预防破坏性疾病。
Many strains of Pseudomonas colonise plant surfaces, including the cherry canker pathogens, Pseudomonas syringae pathovars syringae and morsprunorum. We have examined the genomic diversity of P. syringae in the cherry phyllosphere and focused on the role of prophages in transfer of genes encoding Type 3 secreted effector (T3SE) proteins contributing to the evolution of virulence. Phylogenomic analysis was carried out on epiphytic pseudomonads in the UK orchards. Significant differences in epiphytic populations occurred between regions. Nonpathogenic strains were found to contain reservoirs of T3SE genes. Members of P. syringae phylogroups 4 and 10 were identified for the first time from Prunus. Using bioinformatics, we explored the presence of the gene encoding T3SE HopAR1 within related prophage sequences in diverse P. syringae strains including cherry epiphytes and pathogens. Results indicated that horizontal gene transfer (HGT) of this effector between phylogroups may have involved phage. Prophages containing hopAR1 were demonstrated to excise, circularise and transfer the gene on the leaf surface. The phyllosphere provides a dynamic environment for prophage‐mediated gene exchange and the potential for the emergence of new more virulent pathotypes. Our results suggest that genome‐based epidemiological surveillance of environmental populations will allow the timely application of control measures to prevent damaging diseases.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
5.2
作者：
Gomila, Margarita;Busquets, Antonio;Lalucat, Jorge
通讯作者：
Lalucat, Jorge
影响因子：
2.7
作者：
Hulin MT;Mansfield JW;Brain P;Xu X;Jackson RW;Harrison RJ
通讯作者：
Harrison RJ
DOI：
10.1007/bf00332415
发表时间：
1989-09-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
ELLARD, FM;CABELLO, A;SALMOND, GPC
通讯作者：
SALMOND, GPC
影响因子：
6.7
作者：
Asadulghani M;Ogura Y;Ooka T;Itoh T;Sawaguchi A;Iguchi A;Nakayama K;Hayashi T
通讯作者：
Hayashi T