Characterization and functional analysis of liver-expressed antimicrobial peptide-2 (LEAP-2) in Pelodiscus sinensis
Characterization and functional analysis of liver-expressed antimicrobial peptide-2 (LEAP-2) in Pelodiscus sinensis
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中华鳖肝表达抗菌肽-2 (LEAP-2) 的表征及功能分析
DOI：
10.1016/j.aquaculture.2019.734263
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发表时间：
2019-09
期刊：
影响因子：
4.5
通讯作者：
Lili Wei
中科院分区：
文献类型：
--
作者：
Jianping Fu;Wenjin Liu;Hao Cui;Chunhui Song;Yi Liu;Lili Wei
肝脏表达的抗菌肽-2(LEAP-2)是一种具有两个二硫键的阳离子抗菌肽，在鱼类、两栖动物、鸟类和哺乳动物的宿主防御中发挥着重要作用。在此，我们对中华白对虾的LEAP-2进行了鉴定，并鉴定了其功能。PsLEAP-2的前体由77个氨基酸组成，包括一个22-AA信号肽，一个具有典型切割位点的15-AA前体区(R34XXR37)，以及一个40-AA的成熟肽，其中含有四个保守的半胱氨酸残基，形成两个二硫键(Cys54，60，65，70)。PsLEAP-2在进化上高度保守，其前体/成熟肽与其他已知四足动物的LEAP-2序列有42%-62%/57%-72%的序列同源性。表达分析数据显示，PsLEAP-2仅在肝脏表达。RT-qPCR结果表明，迟缓爱德华氏菌(E.tarda)和甲鱼虹彩病毒(STIV)攻击后，PsLEAP-2的mRNA表达水平均被诱导。在新鲜分离的肝细胞中，PsLEAP-2仅在脂多糖或聚(I：C)刺激后24 h被诱导表达。转导LEAP-2-FLAG基因的鲤鱼上皮瘤细胞对E的摄取减少。与Flag表达载体相比，STIV的病毒产量明显降低。这些结果表明，PsLEAP-2在抵抗细菌和病毒感染的免疫防御中起着重要作用。
Liver-expressed antimicrobial peptide-2 (LEAP-2) is a cationic antimicrobial peptide with two disulfide bonds, and it has been shown to play an important role in the host defence in fish, amphibians, birds and mammals. Here, we characterizeLEAP-2fromPelodiscus sinensisand identify its function. The deduced precursor of PsLEAP-2 was composed of 77 amino acids (aa), including a 22-aa signal peptide, a 15-aa pro-domain with a typical cleavage site (R34XXR37), and a 40-aa mature peptide with four conserved cysteine residues that form two disulfide bonds (Cys54, 60, 65, 70).PsLEAP-2was highly conserved in evolution, and the precursor/mature peptide of PsLEAP-2 showed 42%–62%/57%–72% sequence identity with LEAP-2 sequences from other known tetrapods. Expression analysis data showed thatPsLEAP-2was only expressed in the liver. RT-qPCR results showed that the mRNA expression level ofPsLEAP-2was induced after challenge withEdwardsiella tarda(E. tarda) or soft-shelled turtle iridovirus (STIV). In freshly isolated liver cells, induced expression ofPsLEAP-2was only observed at 24 h after stimulation with LPS or poly(I:C). Epithelioma papulosum cyprinid (EPC) cells transfected with the LEAP-2-Flag plasmid exhibited a reduced intake ofE. tardaand reduced viral yield of STIV compared with cells transfected with the Flag plasmid. These results suggest thatPsLEAP-2plays an important role in the immune defence against bacterial and viral infection.
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影响因子：
4.7
作者：
Gui, Lang;Zhang, Peipei;Zhang, Junbin
通讯作者：
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影响因子：
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作者：
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DOI：
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DOI：
10.3390/ph7060723
发表时间：
2014-06-10
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
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作者：
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通讯作者：
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