Complete in vitro oogenesis: retrospects and prospects.
Complete in vitro oogenesis: retrospects and prospects.
复制标题
完整的体外卵子发生：回顾与展望
DOI：
10.1038/cdd.2017.134
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发表时间：
2017-11
影响因子：
12.4
通讯作者：
Shen W
中科院分区：
文献类型：
--
作者：
Wang JJ;Ge W;Liu JC;Klinger FG;Dyce PW;De Felici M;Shen W
哺乳动物卵子发生的精确控制一直是生殖和发育生物学研究的传统焦点。最近，新的报道介绍了从干细胞体外获得功能性配子的可能性。从干细胞产生功能性配子的潜力在再生医学中具有令人兴奋的应用，但仍然具有挑战性。在雌性哺乳动物中，排卵和受精是少数卵母细胞的特权。事实上，绝大多数由原始生殖细胞（PGC）形成的卵母细胞将经历细胞凋亡或其他形式的细胞死亡。去除发生在生殖细胞囊肿破裂和原始卵泡（PF）池建立期间，在PF阶段的长期休眠期间，或在到达排卵阶段之前通过卵泡闭锁。解决这一限制的一种方法可能是在体外从干细胞产生大量卵母细胞。然而，在体外重现哺乳动物卵子发生和产生可受精卵母细胞是一个复杂的过程，涉及几种不同的细胞类型、精确的滤泡细胞-卵母细胞相互作用、各种营养物质和细胞因子的组合，以及取决于发育阶段的精确的生长因子和激素。 2016年，Morohaku等人发表了两篇论文。和 Hikabe 等人。据报道，体外程序似乎可以有效地重现这些条件，从而完全在培养皿中产生相对大量的卵母细胞，这些卵母细胞是可受精的并且能够在小鼠中产生可存活的后代。本文对这些结果以及主要在试图在体外完全复制卵子发生的小鼠中进行的其他先前工作进行了批判性概述，并考虑了调整这些方法以产生功能性人类卵母细胞的潜力的一些观点。
Precise control of mammalian oogenesis has been a traditional focus of reproductive and developmental biology research. Recently, new reports have introduced the possibility of obtaining functional gametes derived in vitro from stem cells. The potential to produce functional gametes from stem cells has exciting applications for regenerative medicine though still remains challenging. In mammalian females ovulation and fertilization is a privilege reserved for a small number of oocytes. In reality the vast majority of oocytes formed from primordial germ cells (PGCs) will undergo apoptosis, or other forms of cell death. Removal occurs during germ cell cyst breakdown and the establishment of the primordial follicle (PF) pool, during the long dormancy at the PF stage, or through follicular atresia prior to reaching the ovulatory stage. A way to solve this limitation could be to produce large numbers of oocytes, in vitro, from stem cells. However, to recapitulate mammalian oogenesis and produce fertilizable oocytes in vitro is a complex process involving several different cell types, precise follicular cell–oocyte reciprocal interactions, a variety of nutrients and combinations of cytokines, and precise growth factors and hormones depending on the developmental stage. In 2016, two papers published by Morohaku et al. and Hikabe et al. reported in vitro procedures that appear to reproduce efficiently these conditions allowing for the production, completely in a dish, of a relatively large number of oocytes that are fertilizable and capable of giving rise to viable offspring in the mouse. The present article offers a critical overview of these results as well as other previous work performed mainly in mouse attempting to reproduce oogenesis completely in vitro and considers some perspectives for the potential to adapt the methods to produce functional human oocytes.
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影响因子：
3.6
作者：
FINDLAY, JK
通讯作者：
FINDLAY, JK
影响因子：
4
作者：
De Felici, M;Klinger, FG;Lobascio, M
通讯作者：
Lobascio, M
DOI：
10.1007/978-3-319-31973-5_2
发表时间：
2016-01-01
期刊：
Molecular Mechanisms of Cell Differentiation in Gonad Development
影响因子：
--
作者：
De Felici, Massimo
通讯作者：
De Felici, Massimo
影响因子：
4.6
作者：
Hayashi, Katsuhiko;Surani, M. Azim
通讯作者：
Surani, M. Azim
影响因子：
4
作者：
Chen, Ying;Breen, Kelsey;Pepling, Melissa E.
通讯作者：
Pepling, Melissa E.