Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for Plasmodium falciparum transmission.
Enhanced detection of gametocytes by magnetic deposition microscopy predicts higher potential for Plasmodium falciparum transmission.
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DOI：
10.1186/1475-2875-7-66
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发表时间：
2008-04-25
期刊：
影响因子：
3
通讯作者：
Zimmerman PA
中科院分区：
文献类型：
--
作者：
Karl S;David M;Moore L;Grimberg BT;Michon P;Mueller I;Zborowski M;Zimmerman PA
疟原虫感染的红细胞内聚集的疟原虫色素晶体赋予寄生细胞对磁场的敏感性。在这里，我们在巴布亚新几内亚（PNG）的疟疾流行地区评估了这种方法诊断人类疟疾的实用性。患有恶性疟原虫疟疾症状的个体（n = 55）提供了用于常规血涂片（CBS）和磁沉积显微镜（MDM）诊断的样本。进行标准Giemsa染色和光学显微镜检查以评价所有制备物。MDM载玻片上观察到的恶性疟原虫寄生虫血症始终高于（CBS）环中观察到的寄生虫血症（CBS = 2.6 vs. MDM = 3.4%; t检验P值= 0.13），滋养体（CBS = 0.5 vs. MDM = 1.6%; t检验P值= 0.01），（CBS = 0.003 vs. MDM = 0.1%; t检验P值= 0.08）和配子体（CBS = 0.001 vs. MDM = 0.4%; t检验P值= 0.0002）寄生虫血症。与MDM相比，CBS确定的配子体患病率分别从7.3%增加到45%。MDM增加了感染人的恶性疟原虫感染的含疟原虫色素红细胞的检测灵敏度，同时保持了对环期寄生虫的检测。配子体流行率比哥伦比亚广播公司观察到的高五倍，表明与以前的估计相比，巴布亚新几内亚流行地点的疟疾传播潜力更高。
Aggregated haemozoin crystals within malaria-infected erythrocytes confer susceptibility of parasitized cells to a magnetic field. Here the utility of this method for diagnosis of human malaria is evaluated in a malaria-endemic region of Papua New Guinea (PNG). Individuals with Plasmodium falciparum malaria symptoms (n = 55) provided samples for conventional blood smear (CBS) and magnetic deposition microscopy (MDM) diagnosis. Standard Giemsa staining and light microscopy was performed to evaluate all preparations. Plasmodium falciparum parasitaemia observed on MDM slides was consistently higher than parasitaemia observed by (CBS) for ring (CBS = 2.6 vs. MDM = 3.4%; t-test P-value = 0.13), trophozoite (CBS = 0.5 vs. MDM = 1.6%; t-test P-value = 0.01), schizont (CBS = 0.003 vs. MDM = 0.1%; t-test P-value = 0.08) and gametocyte (CBS = 0.001 vs. MDM = 0.4%; t-test P-value = 0.0002) parasitaemias. Gametocyte prevalence determined by CBS compared to MDM increased from 7.3% to 45%, respectively. MDM increased detection sensitivity of P. falciparum-infected, haemozoin-containing erythrocytes from infected humans while maintaining detection of ring-stage parasites. Gametocyte prevalence five-fold higher than observed by CBS suggests higher malaria transmission potential in PNG endemic sites compared to previous estimates.
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影响因子：
2.4
作者：
GRAVES, PM;BURKOT, TR;ALPERS, MP
通讯作者：
ALPERS, MP
DOI：
10.4269/ajtmh.2002.67.555
发表时间：
2002-12-01
影响因子：
3.3
作者：
Mehlotra, RK;Kasehagen, LJ;Zimmerman, PA
通讯作者：
Zimmerman, PA
影响因子：
1.9
作者：
Bellia, PL
通讯作者：
Bellia, PL
影响因子：
3.3
作者：
CATTANI, JA;TULLOCH, JL;CLANCY, R
通讯作者：
CLANCY, R
DOI：
10.4269/ajtmh.2006.75.588
发表时间：
2006-10-01
影响因子：
3.3
作者：
Kasehagen, Laurin J.;Mueller, Ivo;Zimmerman, Peter A.
通讯作者：
Zimmerman, Peter A.