A versatile pH sensitive chondroitin sulfate-PEG tissue adhesive and hydrogel.
A versatile pH sensitive chondroitin sulfate-PEG tissue adhesive and hydrogel.
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DOI：
10.1016/j.biomaterials.2009.12.033
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发表时间：
2010-04
期刊：
影响因子：
14
通讯作者：
Elisseeff, Jennifer
中科院分区：
文献类型：
--
作者：
Strehin, Iossif;Nahas, Zayna;Arora, Karun;Nguyen, Thao;Elisseeff, Jennifer
关键词：
我们开发了一种具有许多潜在生物医学应用的硫酸软骨素-聚乙二醇（CS-PEG）粘合剂水凝胶。硫酸软骨素（CS）链上的羧基用N-羟基琥珀酰亚胺（NHS）官能化以产生硫酸软骨素琥珀酰亚胺基琥珀酸酯（CS-NHS）。纯化后，CS-NHS分子可与伯胺反应形成酰胺键。因此，使用六臂聚乙二醇胺PEG-（NH 2）6作为交联剂，我们形成了通过酰胺键与组织中的蛋白质共价结合的水凝胶。通过改变前体溶液的初始pH，可以控制水凝胶的刚度、溶胀性质和凝胶化动力学。密封/粘合强度也可以通过改变材料的阻尼和储能模量特性来改变。该材料与软骨组织的粘合强度比纤维蛋白胶高10倍。包封或直接接触材料的细胞保持活力和代谢活性。此外，CS-PEG材料在皮下植入大鼠模型时产生最小的炎症反应，并在体外证明了酶降解。这项工作建立了一个粘合剂水凝胶衍生自生物和合成成分，在伤口愈合和再生医学的潜在应用。
We developed a chondroitin sulfate - polyethylene glycol (CS-PEG) adhesive hydrogel with numerous potential biomedical applications. The carboxyl groups on chondroitin sulfate (CS) chains were functionalized with N-hydroxysuccinimide (NHS) to yield chondroitin sulfate succinimidyl succinate (CS-NHS). Following purification, the CS-NHS molecule can react with primary amines to form amide bonds. Hence, using six arm polyethylene glycol amine PEG-(NH2)6 as a crosslinker we formed a hydrogel which was covalently bound to proteins in tissue via amide bonds. By varying the initial pH of the precursor solutions, the hydrogel stiffness, swelling properties, and kinetics of gelation could be controlled. The sealing/adhesive strength could also be modified by varying the damping and storage modulus properties of the material. The adhesive strength of the material with cartilage tissue was shown to be ten times higher than that of fibrin glue. Cells encapsulated or in direct contact with the material remained viable and metabolically active. Furthermore, CS-PEG material produced minimal inflammatory response when implanted subcutaneously in a rat model and enzymatic degradation was demonstrated in vitro. This work establishes an adhesive hydrogel derived from biological and synthetic components with potential application in wound healing and regenerative medicine.
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影响因子：
9.7
作者：
Comolli, Noelle;Neuhuber, Birgit;Fischer, Itzhak;Lowman, Anthony
通讯作者：
Lowman, Anthony
影响因子：
--
作者：
BALAZS, EA;WATSON, D;ROSEMAN, S
通讯作者：
ROSEMAN, S
影响因子：
3.2
作者：
Hayes, Anthony J.;Tudor, Debbie;Hughes, Clare E.
通讯作者：
Hughes, Clare E.
影响因子：
14
作者：
Peyton, Shelly R.;Raub, Christopher B.;Putnam, Andrew J.
通讯作者：
Putnam, Andrew J.
DOI：
10.1016/0019-2791(76)90223-8
发表时间：
1976-01-01
期刊：
IMMUNOCHEMISTRY
影响因子：
--
作者：
RAEPPLE, E;HILL, HU;LOOS, M
通讯作者：
LOOS, M