Structural identification of mouse fecal metabolites of theaflavin 3,3'-digallate using liquid chromatography tandem mass spectrometry.
Structural identification of mouse fecal metabolites of theaflavin 3,3'-digallate using liquid chromatography tandem mass spectrometry.
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DOI：
10.1016/j.chroma.2011.08.056
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发表时间：
2011-10-14
期刊：
影响因子：
--
通讯作者：
Sang S
中科院分区：
文献类型：
--
作者：
Chen H;Parks TA;Chen X;Gillitt ND;Jobin C;Sang S
饮用红茶与许多健康益处有关，包括预防癌症和心脏病。茶黄素是红茶中存在的主要生物活性多酚。不幸的是，有关其生物转化的信息有限。在本研究中，我们使用液相色谱/电喷雾电离串联质谱法，通过分析 MSn (n = 1–3) 谱图，研究了小鼠粪便样品中最丰富、最具生物活性的茶黄素之一茶黄素 3,3'-二没食子酸酯 (TFDG) 的代谢命运。四种代谢物茶黄素、茶黄素 3-没食子酸酯、茶黄素 3'-没食子酸酯和没食子酸被确定为 TFDG 的主要小鼠粪便代谢物。检测并鉴定出茶黄素 3-没食子酸酯、茶黄素 3'-没食子酸酯和 TFDG 的葡萄糖醛酸化和硫酸化代谢物，而不是甲基化代谢物，它们是 TFDG 的次要小鼠粪便代谢物。我们的结果表明 TFDG 可以在小鼠体内被降解。有必要进一步研究在无菌条件下经 TFDG 处理的小鼠中这些代谢物的形成。据我们所知，这是第一份关于 TFDG 在小鼠体内生物转化的报告。
Black tea consumption has been associated with many health benefits including the prevention of cancer and heart disease. Theaflavins are the major bioactive polyphenols present in black tea. Unfortunately, limited information is available on their biotransformation. In the present study, we investigated the metabolic fate of theaflavin 3,3′-digallate (TFDG), one of the most abundant and bioactive theaflavins, in mouse fecal samples using liquid chromatography/electrospray ionization tandem mass spectrometry by analyzing the MSn (n = 1–3) spectra. Four metabolites theaflavin, theaflavin 3-gallate, theaflavin 3′-gallate, and gallic acid were identified as the major mouse fecal metabolites of TFDG. Glucuronidated and sulfated, instead of methylated metabolites of theaflavin 3-gallate, theaflavin 3′-gallate, and TFDG were detected and identified as the minor mouse fecal metabolites of TFDG. Our results indicate that TFDG can be degraded in mice. Further studies on the formation of those metabolites in TFDG-treated mice in germ-free conditions are warranted. To our knowledge, this is the first report on the biotransformation of TFDG in mice.
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DOI：
10.1016/0165-7992(94)90041-8
发表时间：
1994-01-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
SHIRAKI, M;HARA, Y;KAWAKISHI, S
通讯作者：
KAWAKISHI, S
影响因子：
4.7
作者：
Yang, GY;Liu, ZJ;Yang, CS
通讯作者：
Yang, CS
DOI：
10.1038/nrc2641
发表时间：
2009-06
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.2
作者：
Henning, Susanne M.;Aronson, William;Heber, David
通讯作者：
Heber, David
影响因子：
4.2
作者：
Forester, Sarah C.;Waterhouse, Andrew L.
通讯作者：
Waterhouse, Andrew L.