Hopeful monsters: unintended sequencing of famously malformed mite mitochondrial tRNAs reveals widespread expression and processing of sense-antisense pairs.
Hopeful monsters: unintended sequencing of famously malformed mite mitochondrial tRNAs reveals widespread expression and processing of sense-antisense pairs.
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充满希望的怪物：已知畸形螨线粒体tRNA的非预期测序揭示了有义-反义对的广泛表达和加工。
DOI：
10.1093/nargab/lqaa111
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发表时间：
2021-03
影响因子：
4.6
通讯作者：
Sloan DB
中科院分区：
文献类型：
--
作者：
Warren JM;Sloan DB
虽然tRNA结构是分子生物学中最保守和最容易识别的形状之一，但在后生动物的线粒体基因组中经常发现异常的tRNA。几种线粒体tRNAs(mt-tRNAs)的极端退化结构导致了人们对其表达和功能的怀疑。据预测，来自蜘蛛纲超目螨目的螨类具有一些最短的mt-tRNA，完全失去了三叶草般的形状。在植物组织中进行线粒体分离和最近开发的tRNA-seq方法时，我们无意中对一种常见的植物害虫--二尖叶螨Tetranychus urticae的mt-tRNA进行了测序，覆盖范围足够高，足以检测到之前注释的所有tRNA区域。这些结果不仅证实了这些高度分化的tRNAs的表达、CCA拖尾和转录后碱基修饰，而且揭示了多个麻风菌mt-tRNAs的正、反义表达。Mt-tRNA基因的镜像表达已经被假设，但以前并没有证明在任何系统中都是常见的。我们讨论了这些不同的tRNA在翻译和转录成熟途径中作为解码分子和处理信号的功能角色，以及正义-反义对如何为线粒体基因组奇怪的tRNA生物学增加了另一个维度。
Although tRNA structure is one of the most conserved and recognizable shapes in molecular biology, aberrant tRNAs are frequently found in the mitochondrial genomes of metazoans. The extremely degenerate structures of several mitochondrial tRNAs (mt-tRNAs) have led to doubts about their expression and function. Mites from the arachnid superorder Acariformes are predicted to have some of the shortest mt-tRNAs, with a complete loss of cloverleaf-like shape. While performing mitochondrial isolations and recently developed tRNA-seq methods in plant tissue, we inadvertently sequenced the mt-tRNAs from a common plant pest, the acariform mite Tetranychus urticae, to a high enough coverage to detect all previously annotated T. urticae tRNA regions. The results not only confirm expression, CCA-tailing and post-transcriptional base modification of these highly divergent tRNAs, but also revealed paired sense and antisense expression of multiple T. urticae mt-tRNAs. Mirrored expression of mt-tRNA genes has been hypothesized but not previously demonstrated to be common in any system. We discuss the functional roles that these divergent tRNAs could have as both decoding molecules in translation and processing signals in transcript maturation pathways, as well as how sense–antisense pairs add another dimension to the bizarre tRNA biology of mitochondrial genomes.
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影响因子：
4.1
作者：
Chen F;Tang Q;Bian K;Humulock ZT;Yang X;Jost M;Drennan CL;Essigmann JM;Li D
通讯作者：
Li D
DOI：
10.1093/bioinformatics/btp250
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Darty K;Denise A;Ponty Y
通讯作者：
Ponty Y
影响因子：
3.7
作者：
Bushnell B;Rood J;Singer E
通讯作者：
Singer E
影响因子：
14.9
作者：
Jühling F;Pütz J;Bernt M;Donath A;Middendorf M;Florentz C;Stadler PF
通讯作者：
Stadler PF
影响因子：
14.9
作者：
Jühling F;Mörl M;Hartmann RK;Sprinzl M;Stadler PF;Pütz J
通讯作者：
Pütz J