The effect of chlorhexidine on dental calculus formation: an in vitro study.
The effect of chlorhexidine on dental calculus formation: an in vitro study.
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DOI：
10.1186/s12903-018-0517-3
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发表时间：
2018-03-27
期刊：
影响因子：
2.9
通讯作者：
Noiri Y
中科院分区：
文献类型：
--
作者：
Sakaue Y;Takenaka S;Ohsumi T;Domon H;Terao Y;Noiri Y
葡萄糖酸氯己定（CHG）已被证明是有效的预防和控制生物膜的形成。同时，结石形成的增加被认为是相当大的副作用之一。本研究的目的是调查是否矿物质沉积前结石形成后，在早期阶段使用CHG使用在体外唾液相关的生物膜模型。生物膜在MBEC™装置上在含有0.5%蔗糖的脑心浸液（BHI）肉汤中在37 °C下在厌氧条件下显影3天。将生物膜周期性地暴露于每12小时施加1分钟的0.12%CHG，并在含有钙化溶液的BHI中孵育长达2天。分别用原子吸收分光光度法和磷酸盐测定试剂盒测定生物膜中的钙和磷酸盐。使用扫描电子显微镜（SEM）观察形态结构，并使用电子探针显微分析仪（EPMA）分析化学成分。与对照组相比，CHG单次暴露后Ca和Pi的浓度显著增加。反复暴露于CHG浓度依赖性地增加生物膜的Ca沉积，并且Ca的量是对照的5倍。CHG处理的生物膜中的Pi水平显著高于来自对照组的那些（p < 0.05）;然而，暴露次数的影响是有限的。使用SEM和EPMA分析表明，在CHG处理过的生物膜中存在许多含有钙和磷酸盐复合物的簇。在簇的组成分析后，检测到钙的浓度大于磷酸盐。研究结果表明，CHG可能会促进矿物质吸收到生物膜后不久，其使用。有必要在开始使用CHG漱口水之前破坏生物膜，以减少与此程序相关的副作用。病人的管理也很重要。
Chlorhexidine gluconate (CHG) has been proven to be effective in preventing and controlling biofilm formation. At the same time, an increase in calculus formation is known as one of considerable side effects. The purpose of this study was to investigate whether mineral deposition preceding a calculus formation would occur at an early stage after the use of CHG using an in vitro saliva-related biofilm model. Biofilms were developed on the MBEC™ device in brain heart infusion (BHI) broth containing 0.5% sucrose at 37 °C for 3 days under anaerobic conditions. Biofilms were periodically exposed to 1 min applications of 0.12% CHG every 12 h and incubated for up to 2 days in BHI containing a calcifying solution. Calcium and phosphate in the biofilm were measured using atomic absorption spectrophotometry and a phosphate assay kit, respectively. Morphological structure was observed using a scanning electron microscope (SEM), and chemical composition was analyzed with an electron probe microanalyzer (EPMA). The concentrations of Ca and Pi following a single exposure to CHG increased significantly compared with the control. Repeatedly exposing biofilms to CHG dose-dependently increased Ca deposition, and the amount of Ca was five times as much as that of the control. Pi levels in CHG-treated biofilms were significantly higher than those from the control group (p < 0.05); however, the influence of the number of exposures was limited. Analyses using an SEM and EPMA showed many clusters containing calcium and phosphate complexes in CHG-treated biofilms. Upon composition analysis of the clusters, calcium was detected at a greater concentration than phosphate. Findings suggested that CHG may promote mineral uptake into the biofilm soon after its use. It is necessary to disrupt the biofilm prior to the start of a CHG mouthwash in order to reduce the side effects associated with this procedure. The management of patients is also important.
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影响因子：
3.7
作者：
Ohsumi T;Takenaka S;Wakamatsu R;Sakaue Y;Narisawa N;Senpuku H;Ohshima H;Terao Y;Okiji T
通讯作者：
Okiji T
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通讯作者：
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影响因子：
4.3
作者：
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通讯作者：
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影响因子：
6.7
作者：
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通讯作者：
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影响因子：
6.7
作者：
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通讯作者：
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